Chlorophyta, Elodea cyclosis, Paramecium (photo added)

curt0909 · Post by **curt0909** » Mon Jul 02, 2012 11:47 pm

Video on youtube best seen in HD 720p. Nomarski DIC. Nikon 60x 1.40 Planapo. ID of Chlorophyta would be greatly appreciated. Specimen from North East USA http://www.youtube.com/watch?v=z3iqoczT ... ature=plcp

Here is a still of cytoplasmic streaming in Elodea

BJ · Post by BJ » Tue Jul 03, 2012 4:53 am

hi Curt,

another nice video with excellent detail.

I think that your green alga is a non-motile cell of a Chlamydomonas species.

A single basal pyrenoid (great to see the surrounding starch grains so clearly) and a cup-shaped chloroplast put it in the subgenus Euchlamydomonas.

The cell shape and particularly the lack of a protuberance at the apex suggest it may be Chlamydomonas reinhardtii - one of the commonest species in the genus and definitely found in puddles and small ponds.

I often find it difficult to identify organisms from a single photo. With your video and the changes in focus through the cell it is much easier.

thank you for sharing

brian

RogelioMoreno · Post by **RogelioMoreno** » Tue Jul 03, 2012 6:37 am

Very nice video!

Rogelio

Charles Krebs · Post by **Charles Krebs** » Tue Jul 03, 2012 7:52 pm

Curt,

The 720p video looks great.

At the end of the video there are some ciliates and a rotifer where the lighting appears to be somewhat uneven... the middle looks brighter than the edges. Is that how it appears through the eyepieces?

Litonotus · Post by **Litonotus** » Tue Jul 03, 2012 9:11 pm

great video (: the image is also very good.

curt0909 · Post by **curt0909** » Tue Jul 03, 2012 9:40 pm

Brian, thank you for your identification. Your knowledge on the topic is impressive. I prefer to shoot video for that reason. It can provide much more information than stills.

Rogelio, Charles, Litonotus thank you.

Charles, with certain objectives the DIC prisms do not make a good match. I try to hid this by adding a gradient to the opposite side in post.

Reichert Diastar infinity microscope with 1.40 NA DIC condenser
I have 10, 20, 40 reichert plan achromats and a 100x reichert plan fluor 1.30 that give a nice even background DIC image, a 40 reichert neoplan that shows a strong gradient, nikon 20, 40, 60, 100 planapos(noninfinity) that give mixed results. The 60 is a great match. Even when using 30mm ultrawide eyepieces the background is relatively even. The 40 is not a good combination but it gives acceptable DIC if I stop down the condenser. here's an example. The first 2 scenes are done with the nikon 40x plan apo. http://www.youtube.com/watch?v=eeuONqXD ... ature=plcp

arturoag75 · Post by **arturoag75** » Wed Jul 04, 2012 12:48 am

Superb job

arturo

curt0909 · Post by **curt0909** » Wed Jul 04, 2012 9:59 am

Thanks Art

fpelectronica · Post by **fpelectronica** » Wed Jul 04, 2012 11:00 am

Good video!
Francisco

Jacek · Post by **Jacek** » Wed Jul 04, 2012 11:30 am

Beautiful photo and video

curt0909 · Post by **curt0909** » Wed Jul 04, 2012 9:54 pm

Thank you Francisco and Jacek