mesoscale selective plane illumination microscopy: mesoSPIM

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blekenbleu
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mesoscale selective plane illumination microscopy: mesoSPIM

Post by blekenbleu »

Open (GPL v3) hardware and software light-sheet microscopes for large cleared samples (52 x 52 x 102 mm travel range)
https://mesospim.org/
Axially scanned light-sheet microscope with uniform axial resolution of 6.55 um across 13.3mm FOV
standard light-sheet illumination:
Image
mesoSPIM:
Image

The current generation uses Olympus MVX10 and at least 4 lasers >50-150 mW
Image
https://github.com/mesoSPIM/mesoSPIM-ha ... umentation

20 setups world-wide, US$169,600 to 239,600
https://mesospim.org/setups/

More nearly DIY: X-OpenSPIM https://openspim.org/
Metaphot, Optiphot 1, 66; AO 10, 120, and EPIStar 2571
https://blekenbleu.github.io/microscope
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rjlittlefield
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Re: mesoscale selective plane illumination microscopy: mesoSPIM

Post by rjlittlefield »

Lovely -- such a simple idea, such great results!

https://mesospim.org/aslm/#

--Rik
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