Open (GPL v3) hardware and software light-sheet microscopes for large cleared samples (52 x 52 x 102 mm travel range)
https://mesospim.org/
Axially scanned light-sheet microscope with uniform axial resolution of 6.55 um across 13.3mm FOV
standard light-sheet illumination:
mesoSPIM:
The current generation uses Olympus MVX10 and at least 4 lasers >50-150 mW
https://github.com/mesoSPIM/mesoSPIM-ha ... umentation
20 setups world-wide, US$169,600 to 239,600
https://mesospim.org/setups/
More nearly DIY: X-OpenSPIM https://openspim.org/
mesoscale selective plane illumination microscopy: mesoSPIM
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- blekenbleu
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mesoscale selective plane illumination microscopy: mesoSPIM
Metaphot, Optiphot 1, 66; AO 10, 120, and EPIStar 2571
https://blekenbleu.github.io/microscope
https://blekenbleu.github.io/microscope
- rjlittlefield
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Re: mesoscale selective plane illumination microscopy: mesoSPIM
Light sheet microscopy is very cool. I've seen incredible examples of this at Janelia Farm campus of HHMI--drosophila brain. In the above example, what is the fluorescent label for the neurons? Hippocampus looks great. Beautiful work.