I photographed the flowers with the Nikkor 300mm f/4E PF lens mounted on a Nikon D500 camera. The bouquet of flowers was 12 inches wide, so I had to place the front of the 300mm lens 12 feet from the flowers to have all of the flowers in view.
I mounted the camera on a tripod and used live view to manually focus the flowers. I turned the lens focus ring clockwise in small increments to take 8 photos of the flowers, starting with the front of the bouquet. I use the Nikon MC-36 cable release to fire the shutter, but waited a few seconds after turning the lens to reduce vibration. The camera was set at ISO 800, shutter speed at 1/100 second, and the lens at f/4.
Here is the first photo showing the front green flower just about in focus:

Here is the next to last photo showing the top orange Gerbera Daisy just about going out of focus:

Here is the final stack of 8 photos processed in Photoshop CS6:

The final stack is looking very good. This stack was taken 12 feet from the flowers yet, at closer view, I see some inner flower structure in some of the flowers. Lets take a closer view.
Here is a 2 times magnification of the right side of the bouquet:

Sure enough, I see the banana-like florets of the daisies. Lets take a closer look.
Here is a 4 times magnification of the daisies:

Here is another view at 4 times magnification:

And another view at 4 times magnification:

Those banana-like rods around the center disk of the daisies are the trans florets. And around those are the ray florets.
This stack was taken 12 feet from in front of the 300mm f/4E PF lens!
I have to put on my reading glasses and hold these daisies 6 inches from my eyes to clearly see the individual florets. Yet, this lens clearly shows each floret at 12 feet, and the Nikon D500 camera sensor, which is 12 feet 8 inches from the daisies, records each floret! Just amazing!
Just for the heck of it, here are a couple of 8 times magnifications:


All of these magnifications were produced by cropping the original stacked photo in Photoshop CS6, then increasing the cropped image back to the stacked photo original size.
I do not believe I should do any more increased magnifications, otherwise, the photos might be getting pixelated. At 8 times magnification the long side of the photo is 696 pixels, and these are increased back to 5568 pixels. A 16 times mag. it would be 348 pixels, that would be to much.
Leon