DoF is very subjective and depends on the size at which you are viewing the image as well as the importance to our eyes of the detail in the shot. I give formulae based on standard viewing criteria atjzucker wrote:I have been using an online DOF chart to calculate the distance between exposures for my stacks. That's what I used to create the 1mm spaced exposures for this image in the first place. However, when I re-stacked this project using every 4th image, I was changing the spacing from every 1mm to every 4mm and still the focusing stack looks smooth to me. This is telling me that the DOF chart is showing me a more narrow DOF than what is actually required to create a good focusing stack.
Is there an article discussing this in any detail? Obviously, I'd like to make the minimum of exposures for a given stack in order to maintain a smooth and consistent focus.
but any such calculation of DoF is only a guide.