Bread mould
Moderators: rjlittlefield, ChrisR, Chris S., Pau
Bread mould
Hello everybody,
This time bread mould in partially polarised light:
https://live.staticflickr.com/65535/528 ... 9508_o.jpg
Best, ADi
This time bread mould in partially polarised light:
https://live.staticflickr.com/65535/528 ... 9508_o.jpg
Best, ADi
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Re: Bread mould
That's really cool! Are the colors only from polarization or is it also stained?
Re: Bread mould
Hi Jay,
Nothing special but used a combination of partial polarisation
of transmitted light and obliquely reflected light.
Simply point an LED flashlight at a very oblique angle at the slide.
Best, ADi
Nothing special but used a combination of partial polarisation
of transmitted light and obliquely reflected light.
Simply point an LED flashlight at a very oblique angle at the slide.
Best, ADi
- MarkSturtevant
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Re: Bread mould
I really like this one! It grabs me as an appealing example abstract art, but made from recognizable objects. It's often the simple things that give a startling result that has the greatest artistic appeal.
Mark Sturtevant
Dept. of Still Waters
Dept. of Still Waters
Re: Bread mould
Many thanks Mark!MarkSturtevant wrote: ↑Sun May 07, 2023 8:34 amI really like this one! It grabs me as an appealing example abstract art, but made from recognizable objects. It's often the simple things that give a startling result that has the greatest artistic appeal.
- rjlittlefield
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Re: Bread mould
Adalbert, I keep coming back to puzzle over this image.
I am not aware of any way that polarization alone can produce colors like this with mold, and the appearance is quite similar to what is produced by traditional staining such as at https://us.vwr.com/store/product/888159 ... otes-slide .
Was your slide prepared by someone else, and if so can you ask them the process?
--Rik
I am not aware of any way that polarization alone can produce colors like this with mold, and the appearance is quite similar to what is produced by traditional staining such as at https://us.vwr.com/store/product/888159 ... otes-slide .
Was your slide prepared by someone else, and if so can you ask them the process?
--Rik
Re: Bread mould
Hello Rik,
This is a very old slide from Bresser that came with the Biolam.
I cannot see any staining with the naked eye.
Neither with pure transmitted light nor with polarisation.
But with additional side reflected light it has become really colourful.
Best, ADi
This is a very old slide from Bresser that came with the Biolam.
I cannot see any staining with the naked eye.
Neither with pure transmitted light nor with polarisation.
But with additional side reflected light it has become really colourful.
Best, ADi
- rjlittlefield
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Re: Bread mould
Interesting -- thank you for the further information.
I do not recall hearing this effect described anywhere. It may be worth further investigation as an improved method of looking at stained slides, especially old ones.
--Rik
I do not recall hearing this effect described anywhere. It may be worth further investigation as an improved method of looking at stained slides, especially old ones.
--Rik
Re: Bread mould
Hello Rik,
enclosed you will find a picture of the slide: Sorry for the quality. (taken and edited with a mobile phone)
Best, ADi
enclosed you will find a picture of the slide: Sorry for the quality. (taken and edited with a mobile phone)
Best, ADi
Re: Bread mould
Hi Adi,
really I don't understand your technique with this picture. To me it looks like a good but typical bright field microscope image of a stained slide, and at your picture of the whole slide I think that I'm seeing the stained subjects (a low mag. macro picture could confirm it).
Could you gently explain what you did and if possible with images of the setup and the "pure transmitted light nor with polarisation" you said
really I don't understand your technique with this picture. To me it looks like a good but typical bright field microscope image of a stained slide, and at your picture of the whole slide I think that I'm seeing the stained subjects (a low mag. macro picture could confirm it).
Could you gently explain what you did and if possible with images of the setup and the "pure transmitted light nor with polarisation" you said
Pau
Re: Bread mould
Hi Pau,
It looks like that:
1.) Transmitted light with polarisation
https://www.facebook.com/photo/?fbid=77 ... 0269581589
2.) Reflected light from the side (strong torch)
https://www.facebook.com/photo/?fbid=29 ... 0269581589
BTW, I will take some photos of the slide.
Best, ADi
It looks like that:
1.) Transmitted light with polarisation
https://www.facebook.com/photo/?fbid=77 ... 0269581589
2.) Reflected light from the side (strong torch)
https://www.facebook.com/photo/?fbid=29 ... 0269581589
BTW, I will take some photos of the slide.
Best, ADi
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Re: Bread mould
Hi Rik,
Yes, the colors are there, maybe a bit pale, but they are there.
With the sidelight I just lightened them a bit. M6M2, DCR150, Mitu5x
Best, ADi
Yes, the colors are there, maybe a bit pale, but they are there.
With the sidelight I just lightened them a bit. M6M2, DCR150, Mitu5x
Best, ADi
- rjlittlefield
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Re: Bread mould
Unless you made a lot of change in post, that looks to me like a striking improvement from the illumination. It seems much more than "just lightened a bit".
--Rik
Re: Bread mould
Hi Rik,
I always have the polarizing and oblique filters on, but use the side lighting only sometimes.
For me, the sidelight brightened the colours more than the transmitted light.
This is probably due to my implementation of the transmitted light and the use of the two filters.
My way of illumination does not 100% correspond to the standards of microscopy :-) Filters: https://www.facebook.com/photo/?fbid=47 ... 0269581589
Best, ADi
I always have the polarizing and oblique filters on, but use the side lighting only sometimes.
For me, the sidelight brightened the colours more than the transmitted light.
This is probably due to my implementation of the transmitted light and the use of the two filters.
My way of illumination does not 100% correspond to the standards of microscopy :-) Filters: https://www.facebook.com/photo/?fbid=47 ... 0269581589
Best, ADi