Good catch. Yes, the one with 100mm TL will be f/27.Medwar wrote: ↑Mon Jun 14, 2021 1:22 pmIt will be 25x NA 0.55, because of the 100mm TL, so it's F/27, rigth? Still in the diffration territory for this sensor though.rjlittlefield wrote: ↑Fri Jan 08, 2021 5:34 pmAt 50X NA 0.55, you're running at effective aperture f/45 (=50/(2*0.55)). Even for extreme blue wavelength 400 nm, that still puts the diffraction cutoff frequency at 18.2 microns per cycle. Against that, your camera has a sensor that is 3.2 microns per pixel.