Help with Carl Zeiss EPI IV FL Vertical Illuminator

[harisA]

My standard 18 zeiss microscope came with this epi fluorescent illuminator.

I have two questions about it.The illuminator contains a cassete with 2 filters set

1)How i switch from one filter set to another ?

2)Is it possible to modify the illuminator in order to use it as simple epi illuminator (not fluorescent ) . is there a special cassete for this?

[Pau]

1)How i switch from one filter set to another ?

Just pull out the filter assemble after locking the knob. The filter "cubes" are mounted inside a black tube that has two positions.

I have this illuminator but not yet the filters. I think the epi pol assembles don't fit this illuminator but are intended for use on a different one that has an objectives nosepiece and no telan lenses.

[APO]

Switching between filter positions is accomplished by gently pulling the black cylinder out to the second position... The lubricant may be a bit dry so the tug may initially be a bit stiff.

By loosening the forward-facing thumbscrew the whole component holding the filters comes away. Pulling out the 18 mm. circular colored filters is done by pinching the two tabs on the black plastic retaining rings and the 45 degree colored dichroic mirror is accessed by unscrewing the diagonally-split "halves" of the block itself which can then be replaced in one position by a semi-silvered 50/50 dichroic mirror available from Chroma Technology or Omega Filters.

<http://www.chroma.com>

<http://www.omegafilters.com>

Specify the older Zeiss Standard microscope IV-FL fluorescence vertical illuminator and they'll supply the right dichroic.

Both firms can supply semi-silvered mirrors and quality polarizers in different beamsplit ratios and sizes that might prove handy for those building rigs and coaxial lighting set-ups.

If you should ever wish to return this fluorescence vertical illuminator to it's original stock configuration be sure to make a note of which filter goes where, as well as it's correct orientation toward the illumination source, as this is crucial to correct function in fluorescence. Resist the temptation to clean these as well, since the coatings, if they're still intact, are exceedingly delicate and are not intended to be touched by anything.

<http://www.chroma.com/resources-support ... rientation>

[Fredlab]

Hello


(turn the "12" knob)

Dichroic filters are looking like this





For a switch to a simple epi illuminator, i have no idea.

[harisA]

I would like to thank you all for the help provided.Actually i managed to take my first pictures with fluorescent light.