Vitamin C crystal in polarized light

Harald · Post by **Harald** » Tue Jun 14, 2011 11:06 am

Hi there,
This is my result from a weekend of experimenting. This is a single shot, no stacking. Magnification is about 150x. Canon Eos 7D.

Hope you like it

RogelioMoreno · Post by **RogelioMoreno** » Tue Jun 14, 2011 11:12 am

Very nice!

How did you prepare the slide?

Rogelio

Harald · Post by **Harald** » Tue Jun 14, 2011 11:27 am

As I´ve learned C-Vitamin is the same as Acidum Ascorbicum. This was bought in the local pharmacy and dissolved in the ratio 1:50 with water. I put one drop on the microscope slide and waited for about one day for the crystals to form. Water evaporates and the crystals remain on the slide. The most exciting thing is that you never know what you get. You can get 100´s of images on just one slide. Try different magnifications.
I hope this helps and feel free to experiment

Wes · Post by **Wes** » Sun Jul 10, 2011 6:20 am

I have some lab grade ascorbic acid that I have never looked at and this thread inspired me to take a few shots.

BTW what is your microscope? What polarizers does it have?

Harald · Post by **Harald** » Sun Jul 10, 2011 9:28 am

Hi,
I use the Amscope T230A and in this shot I used an 10x Plan objective. I also use a third part camera adapter with my Canon 7D.
The polarizer filter I´ve used is Linear Pola from Hoya. Took one apart and fitted inside the camera adapter and one over the light source, works just fine. As I can turn the filter over the light I have full control..