Cyanobacteria revisited

Images made through a microscope. All subject types.

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Ecki
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Joined: Wed Aug 13, 2008 2:04 am
Location: Cape Town, South Africa
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Cyanobacteria revisited

Post by Ecki »

Dear Forum,

the fact that both Martin and I show pictures of Cyanobacteria under polarized light is pure coincidence.

Since my new scope has POL contrast it is quite understandable that I am playing around with it.

Image

This shows the birefringent cellwalls of a green algae under polarized light. Notice the cyanobacteria sitting on top of it. When I looked at the image I was wondering, why is there no dark background? So I switched the condensor from brightfield to darkfield.

Amazingly now the cyanobactera turned red.

Image

Image

Image
This is a larger cyanobacteria (oscillatoria?) that I accidently crushed.

Of course I had seen pictures of algae fluorescence and this looked familiar. But here we have just a darkfield with POL. This effect is only present with cyanobacteria and there is no red in green algae.

I have tried this with 3 different kinds of cyanobacteria - they turn red. Maybe it is related to the different chlorophyll, I don't know.

Can anybody explain this effect?

I hope you enjoy the pictures as much as I do.
Ecki

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