Scenedesmus

Wim van Egmond · Post by **Wim van Egmond** » Tue Jul 22, 2008 6:29 am

I have not seen this one in the forum yet.

A stack of 8 images.

Wim

ralfwagner · Post by **ralfwagner** » Tue Jul 22, 2008 12:04 pm

Hello Wim,

and I have never seen a better pic of that algae!

Everybody who ever tried to shoot this one knows, that there are several difficulties. First one is to find such a perfectly grown individual, second is to find or put it in a flat position over the whole range and third one is to have all the patience needed to do such a job. Great!

g4lab · Post by **g4lab** » Tue Jul 22, 2008 1:46 pm

Greetings Wim,
I have been a fan of your website since I first got on the web (I think its that long, seems like yours was one of the first microscopy sites I found)

This is a very wonderful image. It seems like a DIC image ,revealing details of the intracellular organelles, and relief (presumably because of the stacking) but with beautiful color too and greater DOF.

Best regards
Gene

BJ · Post by BJ » Thu Jul 24, 2008 3:39 am

Fantastic !

BrianO

rjlittlefield · Post by **rjlittlefield** » Thu Jul 24, 2008 12:00 pm

This is really beautiful!

Manual stack, I presume?

--Rik

Wim van Egmond · Post by **Wim van Egmond** » Fri Jul 25, 2008 12:24 pm

Thanks, my dear micrographriends!

It was stacked with Helicon focus. I let out one image to create a little bit of blur. It thought it shouldn't be too clean:) But I guess it was a bit nonsence to do that.

Yes, Ralph, the small organisms are the hardest but Scenedesmus is quite flat and not so easily damaged. It is a matter of waiting until the slide almost dries out and than make the image. Patience is the key. It would be easier if I would have found a bloom, but there were only a couple in the sample. I picked up the largest viewing the sample under the stereomicroscope.

Gene, Is it that long ago? I guess more than 10 years

Now I am typing along, reminiscing, this was my first article on the web (before I had internet myself)

http://www.microscopy-uk.org.uk/mag/art97/junimg2.html

The interesting thing about drawing microscopic organisms is that you learn far more than by making photographic pictures.

Did I write that?

Have a nice weekend!

Wim

g4lab · Post by **g4lab** » Fri Jul 25, 2008 12:41 pm

Very nice drawing. (in the linked article)
I got on the net in 1999 and the first thing I searched for was microscopy sites. Yours came up. Already with quite good pictures and whimsical sense of humor which I also have enjoyed.
Gene

Jbailey · Post by **Jbailey** » Tue Jul 29, 2008 4:33 pm

Wim:

No one could ask for a better photo of this organism. It is really beautiful!

Jim

bernhardinho · Post by **bernhardinho** » Wed Jul 30, 2008 1:31 am

g4lab wrote: Already with quite good pictures and whimsical sense of humor which I also have enjoyed.
Gene

Hi Gene

would you mind if I correct your spelling here: it should read "WIMsical sense of humor" in that case

Bernhard

dmillard · Post by **dmillard** » Wed Aug 06, 2008 7:36 am

Beautiful, inspiring image - Thank you!

David