box sucker 20x

Images made through a microscope. All subject types.

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Adalbert
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box sucker 20x

Post by Adalbert »


rjlittlefield
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Re: box sucker 20x

Post by rjlittlefield »

Impressive!

What is the specimen, and how was it prepared?

--Rik

Adalbert
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Re: box sucker 20x

Post by Adalbert »

Hi Rik,
Many thanks!
It is Psylla buxi in euparal.
Best,
ADi

rjlittlefield
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Re: box sucker 20x

Post by rjlittlefield »

It looks to me like muscles in the legs are still intact. Is that an illusion, or are they really?

What was the preparation before the euparal?

--Rik

Adalbert
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Re: box sucker 20x

Post by Adalbert »

Hello Rik,
Can it be that it is due to the lighting?
The oblique illumination was combined with reflected light.
LG, ADi

Pau
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Re: box sucker 20x

Post by Pau »

rjlittlefield wrote:
Tue Mar 22, 2022 8:53 pm
It looks to me like muscles in the legs are still intact. Is that an illusion, or are they really?
Likely cross pol would clarify it

Nice image, BTW!
Pau

rjlittlefield
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Re: box sucker 20x

Post by rjlittlefield »

To clarify my question...

When I asked, "What was the preparation before the euparal?", I was hoping to learn what sequence of chemical baths the subject went through.

--Rik

Adalbert
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Re: box sucker 20x

Post by Adalbert »

Hi Pau,
OK, I have just checked it.
The image looks similar, except that the muscles are snow white.
Is that what you meant?
Best, ADi

Adalbert
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Re: box sucker 20x

Post by Adalbert »

Hi Rik,

Not much chemistry was used :-)

They were first swept from the boxwood,
briefly fixed in AFE and then bathed in Isoprop 100%.

Then on the slide, let the alcohol evaporate a little, Euparal and cover glass on it.

AFE = 5% Acetic acid + 5% Formaldehyde + 90% Ethanol

Best,
ADi

Pau
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Re: box sucker 20x

Post by Pau »

Yes, I suppose. Because muscle cells are birefringent due to the parallel protein fibres they are highlighted with cross pol, often with strong interference colours.
Pau

Adalbert
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Re: box sucker 20x

Post by Adalbert »

Hi Pau,
The muscles appear rather white in polarized light:
Image
https://live.staticflickr.com/65535/519 ... 832a_o.jpg
Best, ADi

Pau
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Re: box sucker 20x

Post by Pau »

Adalbert wrote:
Fri Mar 25, 2022 1:57 pm
The muscles appear rather white in polarized light
and very well rendered, I can see the periodic bands striations ,at different places like at the upper right corner.

Interference colours are not typical of a given material but dependable of the thickness together with the RI and the direction of the light. According with the Mitchell-Levy colour interference chart, low 1st order colours are pale grey, so they will appear white against a black background.
Pau

Adalbert
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Re: box sucker 20x

Post by Adalbert »

Hi Pau,
Thank you for the hint about the interference colour chart.
Best, ADi

Adalbert
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Joined: Mon Nov 30, 2015 1:09 pm

Re: box sucker 20x

Post by Adalbert »

Image

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