Rosa blanda Rhizome with Starch
Moderators: rjlittlefield, ChrisR, Chris S., Pau
Rosa blanda Rhizome with Starch
A single Nomarski prism acts (I think) as about a 1/16 wavelength retarder plate. Using just one Nomarski prism and rotating your polarizers to suit can be useful for giving a slight birefringence effect without too-radically altering the apparent colors of your sample. Here for example is a picture of starch granules undergoing enzymatic digestion in the outer cortex of a Rosa blanda rhizome. (Fiixed in FAA, sectioned on a vibratome at about about 30um thick, cleared in Visikol for Plant Biology TM, no stain, imaged at 400x on a Nikon Diaphot TMD using a 40x DIC objective and crossed polarizers with a single Nomarski prism in place behind the objective.) The "maltese cross" interference pattern that you get with starch granules imaged under crossed polarizers is apparent here, but not too striking.
Leonard