Seaweeds fluorescence

Images made through a microscope. All subject types.

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Pau
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Seaweeds fluorescence

Post by Pau »

Few algae autofluorescence stacks
Samples collected few days ago at the Mediterranean rocky shore (Xabia), less than 1m deep.
Both under UV and blue excitation brown and red algae produce the typical red chlorophyll emission while red algae produce very strong yellow emission.

Dictyota sp (Phaeophyta) tallus with unidentified red algae growing on it. UV 365nm LED epifluorescence
A-2021-06-28-18.36.05 ZS DMapModBR.jpg

Corallina sp (Rhodophyta), blue 480nm LED epifluorescence
B-2021-06-29-21.00.41 ZS DMapCorrBR.jpg

Unidentified filamentous green alga (Chlorophyta). UV 365nm LED epifluorescence
C-2021-07-03-21.25.41 ZS PMaxBR.jpg

All taken with Leitz PL Fluotar 10/0.30, custom LED illuminatation on Zeiss WL. EOS 7D afocal 1.6X
Pau

Adalbert
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Re: Seaweeds fluorescence

Post by Adalbert »

very nice :smt041

Luis Carlos
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Re: Seaweeds fluorescence

Post by Luis Carlos »

Very nice set!

Lou Jost
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Re: Seaweeds fluorescence

Post by Lou Jost »

Beautiful. How are you dealing with quenching and bleaching?

Pau
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Re: Seaweeds fluorescence

Post by Pau »

Thanks ADi, Luis Carlos and Lou!
How are you dealing with quenching and bleaching?
In the Rhodophyta yellow emission bleaching is negligible, at least during a 10min set up and stack session, but as you well know chlorophyll photobleaching is important and annoying.
In this cases algae bleaching was somewhat slower than with the typical plants like ferns, in special the Phaeophyta Dictyota
I've shot with the camera in AV mode and corrected contrast and exposure in PP, also I've enabled exposure correction in Zerene, so the images are pretty heavily processed (also lots of dead pixel erasing and strong sharpening)
Pau

Pau
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Re: Seaweeds fluorescence

Post by Pau »

Acetabularia mediterranea , for me the most beautiful alga, an umbrella shaped giant unicellular Chlorophyta alga measuring up to several centimeters. This one is a small sprcimen, about 7mm diameter.
2021-07-05-23.10CorrBR.jpg
UV 365nm with external torch, old Leitz Plan 2.5/0.08, short stack
Pau

Macro_Cosmos
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Re: Seaweeds fluorescence

Post by Macro_Cosmos »

Last one in the first post is my favourite, look at the colours, it's a bit... "hellish?", maybe "gothic" as well, amazing.

Didier_Micro
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Re: Seaweeds fluorescence

Post by Didier_Micro »

Such beautiful colors. Thank you.

ModelZ
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Re: Seaweeds fluorescence

Post by ModelZ »

Nice work. Thanks for showing.

Pau
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Re: Seaweeds fluorescence

Post by Pau »

Thanks Macro_Cosmos, Didier_Micro and ModelZ for your comments.

I didn't see that humble alga diabolic until your comment :lol:
Pau

75RR
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Re: Seaweeds fluorescence

Post by 75RR »

Amazing images! :shock:
Zeiss Standard WL & Wild M8
Olympus E-p2 (Micro Four Thirds Camera)

micro_pix
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Re: Seaweeds fluorescence

Post by micro_pix »

Very nice!

Dave

Robert Berdan
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Re: Seaweeds fluorescence

Post by Robert Berdan »

Beautiful photos nice work.
RB

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