Pleurosigma angulatum (diatom frustule)
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Pleurosigma angulatum (diatom frustule)
An excellent target for resolution tests!
Re: Pleurosigma angulatum (diatom frustule)
Very beautiful. Can you include technical information?
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Re: Pleurosigma angulatum (diatom frustule)
Camera used was a Z6, technique is DIC, at 120x (60x objective with a mag changer's 2x dialled in, so 120x).
No photo-eyepiece, direct projection.
This is a very thin diatom, DIC isn't the best unless it's the high contrast type being used. Phase contrast and COL+POL can yield even better results.
Wemacro for focus stacking as usual, with a focus drive adapted to it (I wrote a post in Equipment Discussions briefing the adaptation).
Re: Pleurosigma angulatum (diatom frustule)
Thanks very much! NA of the objective?
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Re: Pleurosigma angulatum (diatom frustule)
NA of the 60x would be 1.2.
- Robert Berdan
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Re: Pleurosigma angulatum (diatom frustule)
Beautiful photo!
Cheers
RB
Cheers
RB