A view of some common moss from my yard. A splendid target for autofluorescence studies.
Done with DIYed fluoro to Reichert Universal & Zetopan factory epi. R. and Lomo glass, 10-40x. Both epi and DF with 395 & 365 nm leds, sometimes also visble light "painting" with a cardioid. All shortish stacks.
My first pic post here - hope these come out right.
Sphagnia fluoro
Moderators: rjlittlefield, ChrisR, Chris S., Pau
Sphagnia fluoro
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Re: Sphagnia fluoro
Very nicely done!
Dave
Dave
Re: Sphagnia fluoro
Beauties, but do you have any idea why some of the chloroplasts don't seem to be "turned on". Bleaching?
I thought "Royal Blue" was tradiitonal exciter, ~430nm, though I expect the difference would be quantitative only.
I thought "Royal Blue" was tradiitonal exciter, ~430nm, though I expect the difference would be quantitative only.
Chris R
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Macro_Cosmos
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Re: Sphagnia fluoro
Fascinating fluorescence shots, well done!
Re: Sphagnia fluoro
Thanks to all for compliments & comments, I appreciate them.
Details:
Saul: Lomo 10/.4 and 30/.9 WI. Both bright and very much useable in basic fluoro. Latter is a bit finicky to use, razor thin dof, easier a bit stopped down.
ChrisR: Don't really know, could be various things. At the most active growth at the tip the chlrophyl is not yet active even when the chloroplast structures seem to be there. Elsewhere - could be age of the sample or damage. With DF fluoro it is easy to "fry" the sample and I try to use it very carefully. Especially a condenser with a toric lens is unusually efficient for a cardioid - needs restraint.
These here are autumn samples from Scandinavia, so perhaps some of the chloroplast have already let their chemicals go. It was a surprise for me to find out that some biologists (which I'm not) were still arguing what happens to chlorophyt before the leaves drop off. Some seemed to think that it as a complex and expensive molecule is stored somewhere in the stalk/trunk to wait for the spring. Most experts said that it is not the case, but it is just chopped up & recycled. Well, all I can say is that the stalk cross sections that I've seen don't glow in red (under UV, Violet, Royal Blue)...
-Karl
... and one more
Details:
Saul: Lomo 10/.4 and 30/.9 WI. Both bright and very much useable in basic fluoro. Latter is a bit finicky to use, razor thin dof, easier a bit stopped down.
ChrisR: Don't really know, could be various things. At the most active growth at the tip the chlrophyl is not yet active even when the chloroplast structures seem to be there. Elsewhere - could be age of the sample or damage. With DF fluoro it is easy to "fry" the sample and I try to use it very carefully. Especially a condenser with a toric lens is unusually efficient for a cardioid - needs restraint.
These here are autumn samples from Scandinavia, so perhaps some of the chloroplast have already let their chemicals go. It was a surprise for me to find out that some biologists (which I'm not) were still arguing what happens to chlorophyt before the leaves drop off. Some seemed to think that it as a complex and expensive molecule is stored somewhere in the stalk/trunk to wait for the spring. Most experts said that it is not the case, but it is just chopped up & recycled. Well, all I can say is that the stalk cross sections that I've seen don't glow in red (under UV, Violet, Royal Blue)...
-Karl
... and one more
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