Polypodium sporangia fluorescence

[Lou Jost]

I think I didn't post it. Will make some photos and post it in the next day or two in a new thread. If you are dealing with objectives that have good working distances, you can just use a diffused UV flashlight rather than epi lighting. Then all you need is a good visual-cut UV-pass filter on the flashlight and a UV cut filter in the infinity space behind the objective. This has the advantage that there is less autofuorescence in the objective, relative to epi-lighting.

But if I need epi-lighting, I use the ordinary Nikon epi-illuminator with the filtered UV flashlight as light source, and a UV-cut filter above the illuminator. Not as high contrast as a proper fluorescent set-up, but not bad, especially if you don't have access to a proper fluorescence illuminator and filter cube. Some objectives do very badly with this set-up, though.