Diatoms: Star and Triangle shaped

Images made through a microscope. All subject types.

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Macro_Cosmos
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Diatoms: Star and Triangle shaped

Post by Macro_Cosmos »

An ID, even a general one would be great! :D Much appreciated!

Image

Image

Replaced the background, added a drop shadow effect and I like it. 100x, direct projection, DIC.
Thanks for looking.

Pau
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Re: Diatoms: Star and Triangle shaped

Post by Pau »

Triceratium genus, maybe T. favus and T. pentacrinus

Good pictures BTW
The DIC effect seems very faint although very adequate for high contrast subject like diatoms.
Pau

Macro_Cosmos
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Re: Diatoms: Star and Triangle shaped

Post by Macro_Cosmos »

Pau wrote:
Mon Sep 28, 2020 4:41 am
Triceratium genus, maybe T. favus and T. pentacrinus

Good pictures BTW
The DIC effect seems very faint although very adequate for high contrast subject like diatoms.
Prism mismatch :(
I'm using a UIS 100x with UIS2 prisms. Thanks for the IDs!

Lou Jost
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Re: Diatoms: Star and Triangle shaped

Post by Lou Jost »

I think I like the weaker effect.

Luis Barrera Puigdollers
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Re: Diatoms: Star and Triangle shaped

Post by Luis Barrera Puigdollers »

Wow, excellent images for me!!! Could you tell us more about your setup? Direct projection means that you don´t use the trinocular head, nor relay lens and the camera is directly attached over the nosepiece, right? So, the 100x magnification belongs entirely to your objective. The same magnification can be reached with a 40x objective with a 2,5x relay lens but I think there is little sense to use an excellent objective like a splanapo with a common relay lens. Have you tested the difference between the two procedures with regard to image quality? Thanks

Macro_Cosmos
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Re: Diatoms: Star and Triangle shaped

Post by Macro_Cosmos »

Luis Barrera Puigdollers wrote:
Wed Sep 30, 2020 2:07 am
Wow, excellent images for me!!! Could you tell us more about your setup? Direct projection means that you don´t use the trinocular head, nor relay lens and the camera is directly attached over the nosepiece, right? So, the 100x magnification belongs entirely to your objective. The same magnification can be reached with a 40x objective with a 2,5x relay lens but I think there is little sense to use an excellent objective like a splanapo with a common relay lens. Have you tested the difference between the two procedures with regard to image quality? Thanks
Glad you like the photos!

Direct projection means I'm not using a photographic eyepiece. Olympus calls these PE -mag-, such as PE 2.5x, which magnifies by 2.5 times. This means 100x is 100x.
A tube lens is a type of relay lens of course, which I use. I use the U-TLU.

I have not tested the setups. My custom adaptor for the U-SPT just arrived and now I need time to figure out the distances. I do have an excellent 40x objective. NA should stay the same though, so my 40x NA1.0 with 2.5x acts as a 100x NA1.0, which in theory will be inferior to a 100x natively, with an NA of 1.35x. I have the PE 3.3x, PE 2.5x and PE 5x.

75RR
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Re: Diatoms: Star and Triangle shaped

Post by 75RR »

Very nice! Shadow effect works well
Zeiss Standard WL & Wild M8
Olympus E-p2 (Micro Four Thirds Camera)

Olympusman
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Re: Diatoms: Star and Triangle shaped

Post by Olympusman »

Very very nice images.

Mike
Michael Reese Much FRMS EMS Bethlehem, Pennsylvania, USA

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