Subject louse (Phthirus pubis) was examined as follows :
1 Darkfield - Leitz NPL Fluotar 6,3x , unmounted
2 DIC - Leitz 10x/0.45 oil immersion, mounted in euparal
3 Leitz NPL Fluotar 6,3x/0.20 in euparal without coverslip
4 Leitz NPL Fluotar 16x/0.45, mounted in euparal
5 Leitz NPL DIC 25x/0.50, mounted in euparal
6 Leitz NPL DIC 100x/1.30, mounted in euparal
2,3 and 4 were taken with non-dedicated DIC objectives, "pseudo DIC".
Thanks for looking.
Crab Louse in darkfield and DIC
Moderators: rjlittlefield, ChrisR, Chris S., Pau
- Robert Berdan
- Posts: 319
- Joined: Wed Oct 18, 2017 8:58 pm
- Location: Calgary
- Contact:
Nice pictures
Hi Walter very nice pictures thanks for sharing.
Rob
Rob
Thanks both for your positive remarks.
@Houstonx: below a couple of pictures of my DIC -indeed ICT- setup.
Center is an Ortholux II ( the "new" Ortholux) with an eyepiece head designed for 30mm diameter eyepieces. This results in a field number of 24, where the standard FN is 18. So it catches a larger part of the image.
The revolver and condenser are made for DIC, the condenser also has phase contrast stops that I did not use yet. The objectives were mounted in fixed (optimal) position in the revolver in Leitz factory.
Since a couple of months I've been using a mirror house, which makes it possible to switch between flashlight (speedlite) and 10 W Led.
Regards,
Walter
@Houstonx: below a couple of pictures of my DIC -indeed ICT- setup.
Center is an Ortholux II ( the "new" Ortholux) with an eyepiece head designed for 30mm diameter eyepieces. This results in a field number of 24, where the standard FN is 18. So it catches a larger part of the image.
The revolver and condenser are made for DIC, the condenser also has phase contrast stops that I did not use yet. The objectives were mounted in fixed (optimal) position in the revolver in Leitz factory.
Since a couple of months I've been using a mirror house, which makes it possible to switch between flashlight (speedlite) and 10 W Led.
Regards,
Walter
Hi Pau and Saul, thanks for your positive remarks!
@Pau; yes correct it is an afocal setup, identical to the setup with the Orthoplan I posted a couple of years ago in equipment discussions. The camera lens is indeed a pancake and the camera is hanging on the ceiling with a beamer support, so different microscopes can be positioned underneath it.
The eyepiece is a 6,3x Periplan, the same you've been using in your first post in this forum "Diatom in Darkfield" (2010). That was very helpful to me to arrange my setup.
Regards,
Walter
@Pau; yes correct it is an afocal setup, identical to the setup with the Orthoplan I posted a couple of years ago in equipment discussions. The camera lens is indeed a pancake and the camera is hanging on the ceiling with a beamer support, so different microscopes can be positioned underneath it.
The eyepiece is a 6,3x Periplan, the same you've been using in your first post in this forum "Diatom in Darkfield" (2010). That was very helpful to me to arrange my setup.
Regards,
Walter
- Charles Krebs
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- Location: Issaquah, WA USA
- Contact:
Hi Charles, thanks for your positive response!
Regards,
Walter
The body is a rather translucent indeed, small insects are therefore good to combine with darkfield. I do like experimenting with darkfield, next post will be diatoms again.Charles Krebs wrote:Nice set! I'm partial to the darkfield myself, very 3-dimensional and great light light on the upper surface (is the body somewhat translucent?)
Regards,
Walter