Cross Spider In Polarized Light

Perl · Post by **Perl** » Sun Dec 30, 2007 7:45 am

Cross Spider In Polarized Light

Picture taken with Coolpix 4500 via Eyepice
Microscope was Ortholux II wit Pol Util

Regards
Pär Lundqvist
Sweden

Ken Ramos · Post by **Ken Ramos** » Sun Dec 30, 2007 8:36 am

Seems as though we are or have been seeing a lot of "fang" shots here of late on the forums or maybe it is just my phobia of spiders getting in the way

Quite a colorful image and those fangs do look painful.

Perl · Post by **Perl** » Sun Dec 30, 2007 10:34 am

Hi Ken !

But i mostly do Single shoot of the objects only ,
dont like to use the hokus pokus image manupulations
tools and techniques

Do it more in Science way

Regards
Pär Lundqvist
Sweden

rjlittlefield · Post by **rjlittlefield** » Sun Dec 30, 2007 11:29 am

Pär,

Single shot is a great time-saver if what you want to see shows well when squashed flat.

About this picture, it's very colorful but I don't know what to look for in it.

What features are you interested in -- from a science standpoint -- and what information is brought out by the crossed pols?

--Rik

Perl · Post by **Perl** » Sun Dec 30, 2007 12:00 pm

Hi Rik !

Are in to the idea that diffrent chemical compunds
can be identified whit POL Mikroscope and Spectroscope
mounted to the microscope

Like Mineral identification in Standard POL Applications
but this is showing that same can be done on Biological prep to

This particular picture show distrubution of these
Compounds

Regards
Pär

rjlittlefield · Post by **rjlittlefield** » Sun Dec 30, 2007 12:32 pm

Interesting. So are the red areas different from the brown due to different compounds, or different orientations of the same compounds, or just different thicknesses? How about the bright white tips on the fangs?

--Rik

Perl · Post by **Perl** » Sun Dec 30, 2007 1:14 pm

Hi Rik !

Yes Brown , Red and White are diffrent Chemical Compounds

Is show clearly when polarizer are rotated

The diffrent coulor show distrubutions of Chemical Compounds
in the spider , my idea is that what is done in Optical mineralogy
also can be used in Living thing

Some things have been done in the area , but this is not much
explored yet - much most be done in this , but it will sure be
a intressting area in future

Regards
Pär Lundqvist
Sweden

Charles Krebs · Post by **Charles Krebs** » Sun Dec 30, 2007 2:09 pm

Pär,

I'm a big proponent of checking out some of these small "critters" with crossed polarization. Often it does not reveal too much, but sometimes, as in your picture here, it dramticaly delineates certain structures. I generally think of it revealing structural composition, so it is interesting hear that it may be of some use for actually determining some "chemical" composition characteristics as well.

A few subjects I have found interesting with this method can be seen here.

Hope to see more of your images!

Charlie

bernhardinho · Post by **bernhardinho** » Sun Dec 30, 2007 4:47 pm

Hello Pär

nice picture!!

But I'm a bit amazed about your statements there!! Are you doing this professionally? Because I'm certainly willing to learn, but to my knowledge, birefringence in biological objects doesn't have anything to do with chemical compounds!! Apart from the fact that cellulose is optically active, birefringence here is solely due to structural features of the tissues paralleled fibres. Different interference colors are partly due to different thickness of the object and partly to the orientation of the object in reference to the crossed polars ( may be you have heard of so called additional or substractional position).

Best wishes from Germany and a happy new year!!

Bernhard

Raul G · Post by **Raul G** » Sun Dec 30, 2007 7:28 pm

I also believe cross polars on tissue is a great thing, you must remember the calcium oxalate crystals on Paramecium or the chlorochromes on Pediastrum or Scenedesmus.

Cool img Pär !

Perl · Post by **Perl** » Mon Dec 31, 2007 2:41 am

Hi !

This is just an Idea , that i have to look up closer

The preparations are flat mounted between glas

Have read some papers with Pol analysis on bioligical
preparations that work

Pol light have diffrent look then it pass diffternt crystal
structures in preparations , so i think this work on Bilogical stuff to

Do not work with this profesonaly , worked before with EM and
3D analysis on Virus structure

This is just for fun and to expand limits of whats possible
with light microscope , are much in to the tech stuff on microscopy

Will test and work more on this coming year - have some thinking
about analysis that can be done fast and easy with this

Can share my results here if intrests of others in this ??

Best Regards
Pär Lundqvist
Sweden

bernhardinho · Post by **bernhardinho** » Mon Dec 31, 2007 4:18 am

Perl wrote:Hi !

Pol light have diffrent look then it pass diffternt crystal
structures in preparations , so i think this work on Bilogical stuff to

The difference of why doublefraction and birefringence occur in crystalls and in biolocical "stuff" is fundamental!!! May be it is a good idea to read about crystall optics as well

By the way: how many different chemical compounds would you expect in a spider? It is mainly chitin (optically active) and muscle fibres (parallel structure) that cause interference colors!

Bernhard

Perl · Post by **Perl** » Mon Dec 31, 2007 4:29 am

Hi

To see optical diffrences are only half the idea , the other half
is to use a spectroscope to got more info out from the structure

Thinks this is an intressting track , due to its fast and simple
analysis setup - compared to other techniques

Perhaps its a dead end street - but sure worth looking in to

Regards
Pär Lundqvist
Sweden

Mike · Post by **Mike** » Mon Dec 31, 2007 5:23 pm

Hello all,

Not sure how you come to the conclusion the different colors represent different chemicals - could you explain?

Cheers,
Mike

Perl · Post by **Perl** » Tue Jan 01, 2008 5:51 am

Hi

If different chemicals not represent diffrent coulor in Pol light
what is the diffrent Coulor then represent ??

Pär Lundqvist
Sweden

Cross Spider In Polarized Light

Cross Spider In Polarized Light

Crosspolars on Tissue