Thank you Smokedaddy and nanometer!!
Kurt: Thank you for your kind comments.
I am using DIC for my diatom images, not the scientific way to photograph diatoms, but for me the way to show the best details.
For the the two Mastogloias where you can see them in two different levels I had the good luck to have them on both sides in the slide, to take a look inside and from outside. Inside you can see the "septen", and otuside the pores.
All images are stacks, done with the 100x lense with oil.
Very important is a good slide and the correct mountant.
Here it is a slide from Michel Haak, mountant is Pleurax.
BR Anne
(Excuse my bad language, hoepfully you understand)