classic microscopy, stained cut of Casuarina

anne · Post by **anne** » Sat May 18, 2019 4:00 am

Stained cuts of Casuarina:

and in Polarization:

BR
Anne

75RR · Post by **75RR** » Sat May 18, 2019 6:53 am

Beautiful images. Can you give some details on the microtome.

Smokedaddy · Post by **Smokedaddy** » Sat May 18, 2019 9:55 am

Excellent

Jacek · Post by **Jacek** » Sat May 18, 2019 10:22 am

Very nice

ChrisR · Post by **ChrisR** » Sat May 18, 2019 1:54 pm

Impressive staining work, Anne.

Can you share your recipes?

anne · Post by **anne** » Sun May 19, 2019 12:53 am

Hello,
I have to tell you I am only the photographer. This very rare cuts are from the famous Robin Wacker. A friend was so kind to stain them for me and to mount them.
The staining is done with W3A sym 1, a special formulation developed by Robin Wacker and for a long time a secret of him.
You can find it on page 210 here:

https://www.zobodat.at/pdf/Mikrokosmos_95_4_0001.pdf

BR
anne

grgh · Post by **grgh** » Sun May 19, 2019 2:00 am

Anne
wonderful images.

Any chance you could get an English translation of the PDF posted,
looking through the pages and all look interesting, when i try Google translate, I receive notice that the file is to long.

WalterD · Post by **WalterD** » Sun May 19, 2019 2:36 am

Beautiful images Anne. The staining is excellent.
I came across the name Wacker before and... by coincidence am attempting to use a rotary microtome for botanical sections.

Regards,

Walter

RogelioMoreno · Post by **RogelioMoreno** » Thu May 23, 2019 10:31 am

Anne,

Beautiful set! My favorite is #2, what objective?

Rogelio

anne · Post by **anne** » Thu May 23, 2019 1:40 pm

Walter: Thank you!
Rogelio: I used the 20 SPlanApo Olympus. It is a Stitch of 9 Panels. Thank you!
BR
Anne