Pinus sylvestris autofluorescence stack'n stich

JohnyM · Post by **JohnyM** » Thu Feb 14, 2019 8:55 am

31 fields with 6x on sensor.
Flashgun used to minimize photobleach.

micro_pix · Post by **micro_pix** » Thu Feb 14, 2019 10:41 am

Stunning image! Well done.

pbraub · Post by **pbraub** » Fri Feb 15, 2019 12:36 am

This is a very nice image. Thank you.

How did you prepare the sample?

ChrisR · Post by **ChrisR** » Fri Feb 15, 2019 1:48 pm

Super! Did you use a "cube"?

Sumguy01 · Post by **Sumguy01** » Sat Feb 16, 2019 12:07 pm

Very nice.
Thanks for sharing.

WalterD · Post by **WalterD** » Sun Feb 17, 2019 10:49 am

Nice! Image 1 is my favourite, beautiful example of autofluorescence.

JohnyM · Post by **JohnyM** » Mon Feb 18, 2019 6:18 am

Thank you for kind comments.

Answers in order:
-Sample wasnt prepared, it's just cutted on microtome.
-Sure, cube was V-1A, my personal fav. 90% time image looks better than with UV.
-All of them are same image. 2nd and 3rd are just crops from 1st

Robert Berdan · Post by **Robert Berdan** » Mon Feb 18, 2019 3:20 pm

Very nice photographs.

Post by **Saul** » Mon Feb 18, 2019 6:38 pm

carlos.uruguay · Post by **carlos.uruguay** » Mon Feb 18, 2019 6:51 pm

A beauty!!!!!!!!!!!!!!!!!!!!!!!!

Pinus sylvestris autofluorescence stack'n stich

Pinus sylvestris autofluorescence stack'n stich

Beautiful photos