Paramecium bursara

Images made through a microscope. All subject types.

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Olympusman
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Joined: Sun Jan 15, 2012 12:31 pm

Paramecium bursara

Post by Olympusman »

Paramecium bursara

Image
Michael Reese Much FRMS EMS Bethlehem, Pennsylvania, USA

Grahame
Posts: 60
Joined: Tue Sep 30, 2014 11:36 pm
Location: New Zealand

Post by Grahame »

Nice :D
What was the objective lens combo for this ?

Olympusman
Posts: 4587
Joined: Sun Jan 15, 2012 12:31 pm

Paramecium bursara

Post by Olympusman »

It was a 40X objective, focus stacked series. I have been experimenting with illumination effects at high magnifications - mostly off-set illumination, which results in more three-dimensional relief at the cost of lesser resolution. I have a certain home-made spider darkfield stop that when used a 20X objective provides amazing results. When that same stop is used at 40X or higher, by "feathering" the edge of the spider stop can provide an informative cross-illumination at higher magnifications.

Mike
Michael Reese Much FRMS EMS Bethlehem, Pennsylvania, USA

Grahame
Posts: 60
Joined: Tue Sep 30, 2014 11:36 pm
Location: New Zealand

Post by Grahame »

Thanks for that Olympusman,
I didn't have that picked as 40X.
Sounds like a well trained spider.
Happy silly season.

MASZEK
Posts: 75
Joined: Wed Jul 04, 2018 2:14 am
Location: POLAND

Post by MASZEK »

A very interesting shot. You can see a light three-dimensionality. The picture looks like a bas-relief. Nice. :smt114

Beatsy
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Location: Malvern, UK

Post by Beatsy »

It's surprising how much more you can see using oblique-type illumination, though I always find it difficult to photograph. You've captured it well here. I struggle to do oblique on my Zeiss inverted (not enough suitable adjustments in the illumination train) but I (re)discovered oblique on my Nikon Labophot. Just using a hand to shade some of the light coming in from underneath works a treat - and it's quick and easy to move the hand around for different effects.

Olympusman
Posts: 4587
Joined: Sun Jan 15, 2012 12:31 pm

Oblique lighting

Post by Olympusman »

Thanks, everyone. I started experimenting feathering a spider (darkfield) stop at high magnification after finally figuring out high magnification (60X objectives) Rheinberg illumination. The result is a form of the "Limb Effect" which appears to impart a cross-lighting effect.

Mike
Michael Reese Much FRMS EMS Bethlehem, Pennsylvania, USA

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