Vorticella sp. telotroch - in phase contrast (left) and DIC (right). Zeiss Neofluar 40x/0.75 phase objective, Olympus DIC prisms, Sony DSC-V1 camera.
Vorticella telotroch
Moderators: Chris S., Pau, Beatsy, rjlittlefield, ChrisR
Vorticella telotroch
Still far from perfect - but I'm trying to improve my technique. You can see a twisted macronucleus on the left picture and two rings of cilia on the right.
Vorticella sp. telotroch - in phase contrast (left) and DIC (right). Zeiss Neofluar 40x/0.75 phase objective, Olympus DIC prisms, Sony DSC-V1 camera.
Vorticella sp. telotroch - in phase contrast (left) and DIC (right). Zeiss Neofluar 40x/0.75 phase objective, Olympus DIC prisms, Sony DSC-V1 camera.
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Wim van Egmond
- Posts: 826
- Joined: Tue Aug 15, 2006 9:28 am
- Location: Berkel en Rodenrijs, the Netherlands
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Congratulations, Piotr,
That is certainly DIC. If you rotate the camera 45 degrees, the angle of the unevenness of the background will be horizontal. This way you can use the unevennes as a atmospheric effect.
The two images show that all these illuminiation techniques give an artificial image. Each technique enhances different details!
Have you tried one of your radiolaria?
best regards,
Wim
That is certainly DIC. If you rotate the camera 45 degrees, the angle of the unevenness of the background will be horizontal. This way you can use the unevennes as a atmospheric effect.
The two images show that all these illuminiation techniques give an artificial image. Each technique enhances different details!
Have you tried one of your radiolaria?
best regards,
Wim
Thank you, Wim! 
Great tip! I remember this effect from your pictures - with a blue background the image looks like an underwater view.
I am still experimenting with the radiolaria. The prepared slides I am using are quite thick and they somehow don't work very well with the DIC setup. But I will send some results soon!
Great tip! I remember this effect from your pictures - with a blue background the image looks like an underwater view.
I am still experimenting with the radiolaria. The prepared slides I am using are quite thick and they somehow don't work very well with the DIC setup. But I will send some results soon!
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Charles Krebs
- Posts: 5865
- Joined: Tue Aug 01, 2006 8:02 pm
- Location: Issaquah, WA USA
- Contact:
Piotr...
Great to see some new posts from you! The results look good already, and I look forward to seeing more as you "refine" it.
Something to keep you busy over the cold, wet winter.... Ohh...wait... that's what I experience here
...
... you're down there in San Diego!
...never mind
While it's possible to accomplish most of the things many Photoshop plug-ins do without using them, one that I use all the time is something called Photokit.
It is very nice for graduated dodging and burning (left/right/top/bottom/corners) that is sometimes helpful with DIC or oblique lighting (as well as some types of uneven brightfield). Rather unique as it works as a "layer", and the effect can be changed (or masked) as desired. I'm not a big "plug-in" user, but this one has saved me a great deal of time.
Great to see some new posts from you! The results look good already, and I look forward to seeing more as you "refine" it.
Something to keep you busy over the cold, wet winter.... Ohh...wait... that's what I experience here
...... you're down there in San Diego!
...never mindWhile it's possible to accomplish most of the things many Photoshop plug-ins do without using them, one that I use all the time is something called Photokit.
It is very nice for graduated dodging and burning (left/right/top/bottom/corners) that is sometimes helpful with DIC or oblique lighting (as well as some types of uneven brightfield). Rather unique as it works as a "layer", and the effect can be changed (or masked) as desired. I'm not a big "plug-in" user, but this one has saved me a great deal of time.
Charlie - thank you! Yes, the weather down here is pretty good all year round... I miss real Winter already. I'm going back to Europe for short vacation, so I'm going to see some snow. Thanks for the Photokit tip, I will check it out!
Ken - thank you! I hope I can still picture the cilia better. I have been using Sunpak 383 flash with my Sony camera and I'm not sure if the flash duration is short enough to "freeze" them.
Ken - thank you! I hope I can still picture the cilia better. I have been using Sunpak 383 flash with my Sony camera and I'm not sure if the flash duration is short enough to "freeze" them.