Barnacles, auto-fluorescence II

Images made through a microscope. All subject types.

Moderators: rjlittlefield, ChrisR, Chris S., Pau

Charles Krebs
Posts: 5865
Joined: Tue Aug 01, 2006 8:02 pm
Location: Issaquah, WA USA
Contact:

Barnacles, auto-fluorescence II

Post by Charles Krebs »

Hard to believe it has been about a month since I've posted here. Busy summer, lots of family visiting.

There have sure been plenty of shots posted during that time... hopefully I'll get to look them over this week before the next "influx" of family.

I did get a couple of days of "shooting" in, so here are a few new images.

The first 4 images are of "molted" barnacle cuticles (exoskeleton) against some algae collected at the same time. These were done using UV light for natural fluorescence.

The last two show some very young barnacles just starting out attached to a shell. The first is regular light and shows the subject as it appears naturally to the eye. The second is the appearance under UV illumination.



Image

Image

Image

Image

Image

Image

Smokedaddy
Posts: 1954
Joined: Sat Oct 07, 2006 10:16 am
Location: Bigfork, Montana
Contact:

Post by Smokedaddy »

... excellent (as usual), love number 3!

Johann
Posts: 81
Joined: Tue Aug 02, 2016 12:58 am
Location: South Africa
Contact:

Post by Johann »

Absolutely stunning - thanks for sharing

zzffnn
Posts: 1896
Joined: Thu May 22, 2014 1:25 pm
Location: Houston, Texas, USA
Contact:

Post by zzffnn »

"Very cool!" said my 7 year old son, who also likes barnacles.

What was the on sensor magnification for the last two young barnacles photos?

Thank you for sharing, Charles!
Selling my Canon FD 200mm F/2.8 lens

Jacek
Posts: 5357
Joined: Sun Oct 02, 2011 7:00 am
Location: Poland

Post by Jacek »

Beautiful organisms, unfortunately in the Baltic Sea are not present. Excellent photos

carlos.uruguay
Posts: 5358
Joined: Thu Feb 23, 2012 8:05 pm
Location: Uruguay - Montevideo - America del Sur
Contact:

Post by carlos.uruguay »

:smt041


Charles Krebs
Posts: 5865
Joined: Tue Aug 01, 2006 8:02 pm
Location: Issaquah, WA USA
Contact:

Post by Charles Krebs »

Thanks all!
zzffnn wrote:What was the on sensor magnification for the last two young barnacles photos?

Thank you for sharing, Charles!
That would be 5X (Olympus MPLFLN)

Marek Mis
Posts: 2586
Joined: Sat Jul 10, 2010 9:56 am
Location: Suwalki, Poland
Contact:

Post by Marek Mis »

Wonderful images Charlie !

Marek

vasselle
Posts: 1497
Joined: Sun Jan 05, 2014 2:12 am
Location: France

Post by vasselle »

Superbes photos
Microscope Leitz Laborlux K
Boitier EOS 1200d

ChrisR
Site Admin
Posts: 8668
Joined: Sat Mar 14, 2009 3:58 am
Location: Near London, UK

Post by ChrisR »

Did you notice the red "worms" with blue segments (eg west of central barnacle in last image)?
Chris R

discomorphella
Posts: 607
Joined: Sun Oct 01, 2006 7:26 pm
Location: NW USA

Post by discomorphella »

They're all great, but 3 and 4 are my favorites due to the varied autofluorescence colors. Great shots. What ex/em wavelengths did you use?

David

Charles Krebs
Posts: 5865
Joined: Tue Aug 01, 2006 8:02 pm
Location: Issaquah, WA USA
Contact:

Post by Charles Krebs »

discomorphella wrote:What ex/em wavelengths did you use? David
David, The illumination was from two of the 365nm (Nichia) LED flashlights we've discussed elsewhere on the forum. They were fitted with "UV 375 LED Clean-Up Excitation" filters to avoid spillover into blue. The "barrier" filter was a 420nm long pass from Thorlabs (Schott).


ChrisR
Did you notice the red "worms" with blue segments (eg west of central barnacle in last image)?
Those "worms" are diatoms. See the middle diatom in the third image in this post to see what they look like with "normal" light.
http://www.photomacrography.net/forum/v ... php?t=9180



Here is a "blow-up" from the third image posted in this thread:
Image

Post Reply Previous topicNext topic