Barnacles, auto-fluorescence II
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- Charles Krebs
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Barnacles, auto-fluorescence II
Hard to believe it has been about a month since I've posted here. Busy summer, lots of family visiting.
There have sure been plenty of shots posted during that time... hopefully I'll get to look them over this week before the next "influx" of family.
I did get a couple of days of "shooting" in, so here are a few new images.
The first 4 images are of "molted" barnacle cuticles (exoskeleton) against some algae collected at the same time. These were done using UV light for natural fluorescence.
The last two show some very young barnacles just starting out attached to a shell. The first is regular light and shows the subject as it appears naturally to the eye. The second is the appearance under UV illumination.
There have sure been plenty of shots posted during that time... hopefully I'll get to look them over this week before the next "influx" of family.
I did get a couple of days of "shooting" in, so here are a few new images.
The first 4 images are of "molted" barnacle cuticles (exoskeleton) against some algae collected at the same time. These were done using UV light for natural fluorescence.
The last two show some very young barnacles just starting out attached to a shell. The first is regular light and shows the subject as it appears naturally to the eye. The second is the appearance under UV illumination.
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- Charles Krebs
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David, The illumination was from two of the 365nm (Nichia) LED flashlights we've discussed elsewhere on the forum. They were fitted with "UV 375 LED Clean-Up Excitation" filters to avoid spillover into blue. The "barrier" filter was a 420nm long pass from Thorlabs (Schott).discomorphella wrote:What ex/em wavelengths did you use? David
ChrisR
Those "worms" are diatoms. See the middle diatom in the third image in this post to see what they look like with "normal" light.Did you notice the red "worms" with blue segments (eg west of central barnacle in last image)?
http://www.photomacrography.net/forum/v ... php?t=9180
Here is a "blow-up" from the third image posted in this thread: