final closteriuim... for a while at least ;-)

Charles Krebs · Post by **Charles Krebs** » Mon Apr 03, 2017 10:08 pm

Boy do I have thousands of these in my sample containers. I wasn't intending to post any more auto-fluorescence of these, but I had these two comparison (cross-eyed) stereos already shot. The first is solely auto-fluorescence using 450nm excitation. On the second one I added some full spectrum light from underneath. The final three are just a few "regular" shots.

fonakta · Post by **fonakta** » Mon Apr 03, 2017 10:58 pm

The first looks like a colored SEM. Very nice.

Smokedaddy · Post by **Smokedaddy** » Mon Apr 03, 2017 11:35 pm

Being new to this, where do you find Closterium(s)?

arturoag75 · Post by **arturoag75** » Mon Apr 03, 2017 11:38 pm

Impressive

Jacek · Post by **Jacek** » Tue Apr 04, 2017 3:29 am

Super !

carlos.uruguay · Post by **carlos.uruguay** » Tue Apr 04, 2017 5:27 am

Excellent! as usual

curt0909 · Post by **curt0909** » Tue Apr 04, 2017 7:41 am

Wow, Great images. I've never seen such a homogeneous and dense concentration of them before.

Beatsy · Post by **Beatsy** » Tue Apr 04, 2017 10:05 am

Great stuff. I thought the full spectrum back light in the second one would help the stereo effect, but the 3d turned out much clearer in the first one (for me). Love the last hi-mag image too - cracker! How come those little nodules in the end aren't all blurred? They're always bouncing around when I've seen 'em. Flash?

Johann · Post by **Johann** » Tue Apr 04, 2017 10:16 am

Amazing - always a treat no matter how many you submit

hkv · Post by **hkv** » Tue Apr 04, 2017 12:20 pm

Great images! I have hard time with stereo though... must have a defective brain.

Olympusman · Post by **Olympusman** » Tue Apr 04, 2017 1:36 pm

Very nice. It seems whatever fresh water sample you get, you can always find Closterium - whether you want to or not.

Mike

ChrisR · Post by **ChrisR** » Tue Apr 04, 2017 2:36 pm

Super!

Keep trying Håkan.
Hold your thumb 30-35cm in front of your nose and look at it, with a ~60cm screen about 1m away from you, directly behind.
If you're "aware" of three equal-sized rectangles in a row, that's about right.
Practise holding your eyes crossed that amount. Hold the cross, but refocus your eyes on the screen!

Charles Krebs · Post by **Charles Krebs** » Tue Apr 04, 2017 7:02 pm

Thanks all!

Beatsy wrote:How come those little nodules in the end aren't all blurred? They're always bouncing around when I've seen 'em. Flash?

Yes electronic flash. It is a stack, but after stacking all the images I go to "editing" in Zerene and choose a single frame where they look best and use that one just for the little particles. (These crystals are supposedly barium or calcium sulfate, and they vibrate around due to Brownian motion).

Beatsy · Post by **Beatsy** » Wed Apr 05, 2017 1:02 am

Thanks Charles. Another "trick" teased from the master

I'd read about it being Brownian motion but can't help feeling there's more to it than that. If you look at nearby, similar-sized particles in the water, they don't exhibit anywhere near as much movement. Although the motion appears entirely random (Brownian) I wonder if some other mechanism disturbs the internal fluid too. Or perhaps the crystals are especially light because they're hollow. Or something?

Olympusman · Post by **Olympusman** » Wed Apr 05, 2017 1:13 pm

Or maybe the issue is that the Barium crystals are heavy which may account for the speed of the Brownian motion. Also, if you are focus stacking rapidly, you may strike a position where the crystals had not moved significantly between frames, and so the out of focus frames are not stacked..

Mike