and another marine ciliate

René · Post by **René** » Tue Feb 07, 2017 2:20 am

From an experiment a couple of years ago: typical ciliates from a brackish envirioment, possibly Diophrys. Lugol preservation, dehydrated in ethanol and mounted in Euparal. Size ca. 85um, DIC, 20x/0.7 splanapo.

Best wishes, René

Bruce Taylor · Post by **Bruce Taylor** » Tue Feb 07, 2017 5:44 am

Nice ciliate.

btschumy · Post by **btschumy** » Tue Feb 07, 2017 6:43 am

Rene,

Nice series of images. They were so perfectly set up for focus stacking and I wanted to try some new stacking software that I couldn't help myself.

Here they are stacked with no further processing.

http://www.photomacrography.net/forum/u ... edpg_2.jpg

René · Post by **René** » Tue Feb 07, 2017 8:55 am

Thank you guys! I was hoping for a positive identification Bruce

Thanks for the effort on stacking btshumy. I'm always a bit disappointed by my own efforts on stacking brightfield images, but your image does give a good first-view feeling.

René

Bruce Taylor · Post by **Bruce Taylor** » Tue Feb 07, 2017 9:39 am

René wrote:Thank you guys! I was hoping for a positive identification Bruce

I agree that Diophrys is most likely.

The 3 caudal cirri (at the posterior of the cell, right side) are somewhat damaged, but in the right spot for Diophrys. We don't have a great view of the transverse cirri, but there seem to be 5 of them, which would rule out Paradiophrys. I don't see dorsal cilia, so it's probably not Diophryopsis (unless they're below the focal plane).

René · Post by **René** » Tue Feb 07, 2017 9:58 am

Thank YOU Bruce!

Wim van Egmond · Post by **Wim van Egmond** » Tue Feb 07, 2017 12:47 pm

Really spectacular for a preserved ciliate. Well done René! I will try to collect samples for you and will make sure I use the lugol well. :-)

Wim

Jacek · Post by **Jacek** » Wed Feb 08, 2017 2:30 pm

Very nice

carlos.uruguay · Post by **carlos.uruguay** » Sat Feb 11, 2017 12:57 pm

Nice!