Darkfield Reflected Rheinberg

Images made through a microscope. All subject types.

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mtuell
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Darkfield Reflected Rheinberg

Post by mtuell »

Earlier, I posted a "DRR" image of pollen with 100x BD Plan darkfield reflected Rhienberg, where I placed colored filters over the three sectors of the darkfield objective. Here are some more examples of the technique, here with the 10x BD Plan.

First (my very first one, actually) is a focus stacked image of the very tip of a glass prism (a cornercube, specifically) where the polished surfaces were represented as black. The interesting parts are the ground bevels, where you can see the grinding pits, and with the three-sector Rhienberg, you can tell the local slope directions.

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Next up is a single exposure (not stacked) of a moth wing, showing the scales. Ones that had come loose were especially prone to diffraction, so showed up nicely.

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And finally, two different focus positions of fossilized radiolaria (dry mounted on black paper). The first is an attempt to focus on one of the fossils, and the second is focused on the background.

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I also looked at fresh moss, but it was too 3-D, and focus stacking didn't work too well. So, instead, I made a through focus movie (.gif, 10 MB), which can be accessed at https://lavinia.as.arizona.edu/~mtuell/ ... inberg.gif.

For those with BD/ED/Epi style darkfield objectives, it is pretty easy to do this technique. I used different colors of shim-stock, since I had some, but any transparent colored filters that you can cut into little crescents should work.

Enjoy!
Mike

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Post by Jacek »

Very nice

GaryB
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Post by GaryB »

Love it! It takes on a very surreal look, far more colorful and dramatic than brightfield Rheinberg.

carlos.uruguay
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Post by carlos.uruguay »

Very beautiful
The 3D image excellent!
How did you the 3D movie?

mtuell
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movie

Post by mtuell »

Thanks, everyone!

To make the movie, I just took 22 pictures with varying focal positions, brought them into Photoshop and did an animation. Then saved it to a .gif. I did the same thing with 18 images with a rotating polarizer with reflected light DIC of a bare CCD chip. I'll post that one tomorrow, I guess.

Mike

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Post by carlos.uruguay »

Super!

Charles Krebs
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Post by Charles Krebs »

Keep exploring with this technique.... seems to have the potential for lots of nice images (as seen here).

mtuell
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Post by mtuell »

The first image (cornercube) needs a new caption - inspired by Steve (nanometer).

One Point Twenty-One Jigga-Watts!!!! :lol:

(It's the "Flux Capacitor" from Back to the Future, if that didn't ring a bell!)

Mike

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Post by rjlittlefield »

Mike, thank you for posting the focus stack and letting me play with it.

I used Photoshop CS5 to save out all the layers of your GIF as separate files, then ran those through Zerene Stacker.

Here are the results...

Basic PMax output:

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Animated GIF showing synthetic rocking, 7 frames spanning +-3% shift. It pauses at each center position (0% shift).

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Stereo pair, crosseyed, at +-3% shift:

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Stereo pair, higher resolution: http://janrik.net/MiscSubj/2016/mtuell_moss_20160909/2016-09-09-11.16.36 ZS stereo_000.jpg

This form of illumination is very interesting!

--Rik

mtuell
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Post by mtuell »

Rik,

Thanks for your efforts! It appears that I'll have to invest in Zerene as the funds become available! That really did a superb job compared to my attempts with Focus Stacker. FS is cheap and has done OK with some scenes, but has made a mess of several others. Kudos!

Thanks again!

I hope others with BD epi setups will try this simple illumination technique!

Mike

mtuell
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Post by mtuell »

OK, so DRR (darkfield reflective Rheinberg... try to keep up! :wink: ) is descriptive, but drab. If this actually is a new technique, let it be called (ahem... drum roll please!) :shock:

Disco Lights Illumination

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These are obviously not through a microscope. It is my Nikon D3300, hand-held, with no flash used, with an old manual Tamron 80-210 Tele/Macro from about 3 feet away.

GaryB
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Post by GaryB »

DLI is officially christened

:D

Image

Couldn't resist.

mtuell
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DLI

Post by mtuell »

Gary, that's perfect! Bravo!!!

It does remind me that I realized that this technique can be extended into the macro-scale world. If you got some stage lights with gels, or for smaller objects, different colored LEDs, or small lights with filters, you could set up an arrangement, with your camera above, where you came in from around the edges with different lights... same idea. I haven't tried it yet, but it should work! :idea:

I also realized, but haven't tried to implement, that under certain conditions, such as no focus stacking, but well focused, one should be able to infer the 3D topology from a single image. This would be done by looking at the three color channels (RGY in this case) and doing a vector decomposition to determine the local slope at each pixel. Integrate it, and viola, a topo map! I'm going to see what I can accomplish in this area this weekend, I hope.

Mike

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Post by carlos.uruguay »

Fabulous conversation!! thanks for sharing!

GaryB
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Post by GaryB »

first go of slide mount bubble taken on a webcam.


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