Color Heine vs Regular Heine

Brian Matsumoto · Post by **Brian Matsumoto** » Thu Jun 23, 2016 9:42 am

I've been experimenting with the color Heine condenser and I previously showed Rheinberg and color phase contrast images. The primary purpose of the condenser is, I believe, to generate color phase contrast. Here is a comparison between the condenser being used to generate a phase contrast image and then a color phase contrast image. If anyone has an instruction manual for this condenser, I would be most appreciative for leads on how to obtain it.

zzffnn · Post by **zzffnn** » Thu Jun 23, 2016 9:54 am

Thank you for sharing. The color Heine does give more contrast.

Very nice images! Were they taken with a high NA PV objective?

I heard only 300 of those were ever produced? Member "Perl" sold one previously: http://www.photomacrography.net/forum/v ... hp?t=30571
Maybe he has some clues?

The color Heine has two variable lighted ring, correct? How does it look from objective back lens, say, for the color phase?

Saul · Post by **Saul** » Thu Jun 23, 2016 10:11 am

http://microscope.database.free.fr/Acce ... denser.pdf

Brian Matsumoto · Post by **Brian Matsumoto** » Thu Jun 23, 2016 10:19 am

When you look at the back focal plane of the objective with a phase telescope, you will see two glowing concentric rings. If the filter is installed, the inner ring glows green and the outer ring glows red. The inner ring can be sized to fit the phase ring of a phase objective. The outer ring can be enlarged so it disappears from the back focal plane. In other words, this cone of light lies outside the N.A. of the objective. So, in essence you have a mix of phase contrast (green) and darkfield (red). If you displace the inner ring so that is not coincident with the phase ring, then you achieve what is the equivalent of Rheinberg illumination.

Most of my experiments have been restricted to an Apo N.A. 0.70 Pv objective by Leitz. Eventually, I will start working with the 70x N.A. 1.15 Pv objective and use the oil cap with the condenser. Presently, I am experimenting with different color combinations. For Rheinberg illumination my best results have been obtained with the 25x N.A. 0.50 objective.