Limnesia, Unionicola, Arrenurus

Jacek · Post by **Jacek** » Sun May 01, 2016 12:02 am

Sumguy01 · Post by **Sumguy01** » Sun May 01, 2016 8:11 am

Very nice set.
Thanks for sharing.

vasselle · Post by **vasselle** » Sun May 01, 2016 9:40 am

Bonjour
Très belles séries images
Cordialement seb

carlos.uruguay · Post by **carlos.uruguay** » Sun May 01, 2016 10:01 am

As always great!

_Michal_ · Post by **_Michal_** » Sun May 01, 2016 10:23 am

Fantastic shots

Pau · Post by **Pau** » Sun May 01, 2016 10:36 am

Great set, something usual with you

. The lateral view #5 is IMO the most original.

Please,
- what excitation wavelength did you use for this fluorescence images? (light source and excitation filter if you know it)
- just autofluorescence or any fluorochrome dye?

Jacek · Post by **Jacek** » Sun May 01, 2016 2:05 pm

Thank you very much

All photos autofluorescence, photos 1-4 (excitation filter old zeiss UG1, filter barrier GG9 (400 nm)).
Photo 5 (cube Leica: ex. 365, mirror 400, bar. 420).
Source of light - led with a single point of light 3W UV (365 nm).

(old zeiss filter)

(cube Leica)

Pau · Post by **Pau** » Sun May 01, 2016 2:48 pm

All photos autofluorescence, photos 1-4 (excitation filter old zeiss UG1, filter barrier GG9 (400 nm)).
Photo 5 (cube Leica: ex. 365, mirror 400, bar. 420).
Source of light - led with a single point of light 3W UV (365 nm).

Thanks so much!

Another question, what exposure time and ISO setting you're obtaining with you 3W LED? (I'm most interested because I'm in the way of buying LEDs for autofluorescence)

carlos.uruguay · Post by **carlos.uruguay** » Sun May 01, 2016 10:29 pm

Very very nice!

Jacek · Post by **Jacek** » Sun May 01, 2016 11:09 pm

Thanks Carlos

Exposure time: 1 s.
I use compact camera, 1 s is the maximum exposure time, but the camera does not have manual settings.
ISO: 125-400
at 400 I already have a big noise for a single photo ok, but stacks the unfit.

Pau · Post by **Pau** » Mon May 02, 2016 3:39 am

Exposure time: 1 s.
I use compact camera, 1 s is the maximum exposure time, but the camera does not have manual settings.
ISO: 125-400

This seems strong autofluorescence!
Very useful info for me.
Thanks again!

Always amazed by the excellent results you obtain with such a simple camera.

Marek Mis · Post by **Marek Mis** » Mon May 02, 2016 5:10 am

All the images are very good, but the most impressive for me are the 5 th and the last one. The last one is simply beautiful !

What software have you used for these images, HF or ZS ?

Marek

Jacek · Post by **Jacek** » Tue May 03, 2016 3:46 am

Thanks

1 - 14X HF_B
2,3 - single image
4 - 15X Z. PMax
5 - 13X HF_C
6 - 37X Z. PMax
7 - 28X Z. Pmax