Euglenoid probably Lepocinclis oxyuris

Cactusdave · Post by **Cactusdave** » Sun Feb 14, 2016 2:24 pm

A nice obliging Euglena, possibly E. spirogyra in a mixed algal culture. This individual rolls repeatedly on its axis giving a good view of the internal structure including the chloroplasts and paramylon bodies. Nikon Diaphot DIC using a Nikon X40 plan DIC objective . Canon EOS 5DMk2 camera. Magnification on sensor X100.

See edit of title and Bruce Taylor's comment on I.D.

https://vimeo.com/155313265

Jacek · Post by **Jacek** » Sun Feb 14, 2016 2:57 pm

Very nice

Cactusdave · Post by **Cactusdave** » Mon Feb 15, 2016 2:20 am

Thanks Jacek.

Bruce Taylor · Post by **Bruce Taylor** » Mon Feb 15, 2016 5:37 am

Nice!

It is likely to be Lepocinclis oxyuris, if the size is in the ballpark. Euglena spirogyra (= Lepocinclis spirogyroides) would have a textured pellicle (same goes for Lepocinclis fusca, which is otherwise quite similar to your creature).

Cactusdave · Post by **Cactusdave** » Mon Feb 15, 2016 10:04 am

Thanks Bruce. I have edited the title to reflect your suggestion of ID.

fpelectronica · Post by **fpelectronica** » Mon Feb 15, 2016 11:25 am

Nice video

Charles Krebs · Post by **Charles Krebs** » Mon Feb 15, 2016 6:37 pm

Dave,

Looks like video has you hooked! A lot going on in that slide, can't wait for my collection locations to get more productive.

What ISO do you need to to set on the camera when doing video with a 40X on your Diaphot?

Cactusdave · Post by **Cactusdave** » Tue Feb 16, 2016 3:08 am

Thanks Charles, yes I've enjoyed doing some video of late. Some of the videos I've seen on this site have been very inspiring. This was a mixed algae culture I obtained from a scientific supply house called Sciento in the UK, a sort of mini version of Carolina Biological that you have in the USA. They are a good source of material in the Winter months. I have kept their algae cultures going for months on a windowsill.

What ISO do you need to set on the camera when doing video with a 40X on your Diaphot?

Mostly I have it on ISO 1600. The full frame Canon EOS 5DMk2 has an excellent noise profile at high ISO. ISO 3200 is quite doable if needed. I really like the 5DMk2 for video. The menu for video control is rather buried, and it's not even totally obvious how to start videoing with this camera. No one touch dedicated video button, but when you get to the right place there is good manual control over parameters like ISO. Most of the 'one button video' cameras default to auto ISO which can be very noisy, and any manual options are again buried deep in the menus.

carlos.uruguay · Post by **carlos.uruguay** » Tue Feb 16, 2016 5:38 am

Very nice video

Cactusdave · Post by **Cactusdave** » Tue Feb 16, 2016 1:30 pm

Thanks Carlos.