Thecamoeba striata (amoeba)

75RR · Post by **75RR** » Sat Nov 14, 2015 5:26 am

40x, Phase, Olympus E-p2

Almost missed this one. Not only is it quite small but even in oblique it is very hard to see.
An ideal Phase subject one might say.

https://vimeo.com/145713400

carlos.uruguay · Post by **carlos.uruguay** » Sat Nov 14, 2015 6:42 am

Interesting.
Thanks for sharing

zzffnn · Post by **zzffnn** » Sat Nov 14, 2015 7:08 am

Very nice video!
Have you tried oblique + phase? Phil used them together and showed photos previously at the other forum. They look even better than phase alone, at least to my eyes. There would be more light though.

75RR · Post by **75RR** » Sat Nov 14, 2015 7:17 am

Have you tried oblique + phase?

Not sure how that would work. Phase + UGF you mean?

zzffnn · Post by **zzffnn** » Sat Nov 14, 2015 8:02 am

75RR,

I meant phase + oblique. You don't have to offset phase ring to get oblique.

You can center and focus phase as you normally do. Then add DIY oblique by partially blocking the light entering condenser, at any point below the condenser, with any means. Carlos does it with a coin over his field diaphragm. I do it with a glass slide partially blocked with black tape (then I Blutak the slide under my condenser - Blutak and slide can be moved back and forth to adjust oblique).

I would highly appreciate it, if you can give oblique+phase a try and comment (report findings) on my contrast comparison thread over at the other forum. I don't have phase myself, so I cannot do the comparison myself.

I don't think UGF would look any different than oblique, when either is combined with phase. Condenser phase ring would only leave a hollow ring of light going up, so when UGF is used you probably would only see its oblique part (gradient part will be blocked out by phase ring and not produce any effect).