Thanks for the comments all. To answer your questions...
1. I use Debe's fixative to attach the diatoms to the coverslip while arranging. Thanks to forum-member Raymond for the recommendation as I was using the fixative after Solliday before that, with mixed results. (
http://www.diatomsireland.com/diatom-mo ... adhesives/)
2. I mount the finished arrangement in zrax
3. My micromanipulator was inspired by forum-member Raymond too, in his article at
http://www.microscopy-uk.org.uk/mag/art ... atoms.html. I use a stereo zoom instead of a compound microscope and usually work at 40x but will go up to 100x for very small diatoms. The hand-pulled glass needle just moves up and down and the slide is moved around by hand for picking and placement. Picture of my rig below. Note: I improved vastly on Raymond's design by attaching the straightened paper clip with epoxy instead of a clothes peg
Then just lots of patience and practice.
