Daphnia Details in Darkfield

Charles Krebs · Post by **Charles Krebs** » Sat Jan 17, 2015 4:10 pm

Sorry... just had to use the alliteration in the title

Unfortunately not much time on the microscope lately, but I did come across some daphnia. I wanted to do these with darkfield primarily because of the eye. With brightfield or DIC the middle part of the eye becomes primarily a dark, detail-less "blob". With darkfield you can see much more interesting detail. Live specimens with electronic flash, 10/0.40 and 20/0.70 objectives. Olympus stand with Nikon 0.95-0.80 dry darkfield condenser.

sebba28 · Post by **sebba28** » Sat Jan 17, 2015 6:18 pm

Very nice,

Jacek · Post by **Jacek** » Sat Jan 17, 2015 11:53 pm

SUPER !

escocat · Post by **escocat** » Sun Jan 18, 2015 3:04 am

Eye and antennas are very nice.

Welcome back Charles

arturoag75 · Post by **arturoag75** » Sun Jan 18, 2015 3:10 am

Great job again

carlos.uruguay · Post by **carlos.uruguay** » Sun Jan 18, 2015 4:31 am

Excellent

Wim van Egmond · Post by **Wim van Egmond** » Sun Jan 18, 2015 6:57 am

They are beauties and I hope you will be able to find more time for microscopy!

Wim

Marek Mis · Post by **Marek Mis** » Sun Jan 18, 2015 9:14 am

Perf4ect ! The details are incredible, especially if about the eye.

Marek

piermicro · Post by **piermicro** » Sun Jan 18, 2015 9:54 am

Very,very nice.
Incredible.

fpelectronica · Post by **fpelectronica** » Sun Jan 18, 2015 12:52 pm

Raaj · Post by **Raaj** » Sun Jan 18, 2015 3:09 pm

Beautiful images, Charlie - the detail, the acuity and the colors are excellent. You continue to inspire!

Cheers,
-raaj

Olympusman · Post by **Olympusman** » Sun Jan 18, 2015 3:36 pm

Love the eyespot details.

Mike

discomorphella · Post by **discomorphella** » Sun Jan 18, 2015 5:51 pm

They are all great shots, but my favorite is the final image, with the delicate glass-like branches. I wonder if they are birefringent, i.e. how would they look with POL?

David

vasselle · Post by **vasselle** » Sun Jan 18, 2015 11:06 pm

Very nice photos

pierre · Post by **pierre** » Sun Jan 18, 2015 11:18 pm

Impressive