Geometrid moth proboscis

blepharopsis · Post by **blepharopsis** » Wed Dec 24, 2014 1:40 pm

Calcofuor White + Congo Red, Zeiss LSM 710, 10x NA 0.45, 405, 488, 562 nm laser lines

rjlittlefield · Post by **rjlittlefield** » Wed Dec 24, 2014 1:58 pm

Beautiful!

Any chance of showing us some high mag 3D of the structures that link together the left and right halves??

(See the thread HERE for some of my own less than stellar attempts, and interesting references posted by other people.)

--Rik

blepharopsis · Post by **blepharopsis** » Wed Dec 24, 2014 2:05 pm

rjlittlefield wrote:Beautiful!

Any chance of showing us some high mag 3D of the structures that link together the left and right halves??

(See the thread HERE for some of my own less than stellar attempts, and interesting references posted by other people.)

--Rik

Thanks, Rik! Wow, it's amazing what you can do with MPE-65 and image stacking! I definitely have to start tinkering with step motors and macro rails and whatever it is that makes images like yours possible.

I have to dig out the original stacks, I think imaged the proboscis with a 20x NA 0.8 too. Hope I saved the data...

vasselle · Post by **vasselle** » Wed Dec 24, 2014 3:25 pm

Hello
Very nice
Cordialement seb

carlos.uruguay · Post by **carlos.uruguay** » Wed Dec 24, 2014 3:26 pm

Very nice!

Olympusman · Post by **Olympusman** » Wed Dec 24, 2014 3:26 pm

Beauty!

Mike

RogelioMoreno · Post by **RogelioMoreno** » Wed Dec 24, 2014 3:27 pm

Nice!

Rogelio

rjlittlefield · Post by **rjlittlefield** » Wed Dec 24, 2014 6:13 pm

blepharopsis wrote:Wow, it's amazing what you can do with MPE-65 and image stacking!

I wish! But most of my images in that thread were shot with a Nikon M Plan 40X NA 0.50 ELWD, stacked at 1 micron.

The setup is kind of like a bargain-basement version of that CLSM you use -- just missing the C, the L, the S, and, uh, most of the M...

I would love to see details on the proboscis at 20X NA 0.8 !

--Rik

arturoag75 · Post by **arturoag75** » Thu Dec 25, 2014 2:51 am

Impressive

fpelectronica · Post by **fpelectronica** » Thu Dec 25, 2014 4:18 am

Cactusdave · Post by **Cactusdave** » Fri Dec 26, 2014 10:08 am

I took some images of this subject with DIC a while back http://www.photomacrography.net/forum/v ... 9562#89562
but they are pretty tame in comparison with that lovely image of yours. Its a topic I keep meaning to go back to.

Jacek · Post by **Jacek** » Fri Dec 26, 2014 1:52 pm

Very nice

pwnell · Post by **pwnell** » Sat Dec 27, 2014 11:41 am

Excellent photo.

Geometrid moth proboscis

Geometrid moth proboscis

Moth probocis