Pediastrum - each one unique, like a snowflake :)

blepharopsis · Post by **blepharopsis** » Sun Dec 21, 2014 2:11 pm

Collection of Pediastrum from the pond right in front of Janelia institute. I used the same technique to stain the cell wall (Calcofluor White) and image the specimens (405, 488 and 562 nm laser lines); the variations in the appearance of individual colonies, or even individual cells within them, was a bit of a surprise for me.

Imaged with Zeiss LSM 710, 40x NA 1.3

carlos.uruguay · Post by **carlos.uruguay** » Sun Dec 21, 2014 2:47 pm

Amazing!

RogelioMoreno · Post by **RogelioMoreno** » Sun Dec 21, 2014 2:49 pm

Beautiful, I love it.

Rogelio

discomorphella · Post by **discomorphella** » Sun Dec 21, 2014 5:27 pm

Images are spectacular as usual. These are unfixed right? I wonder if the variation in the fluorescence is due to some of the segments being alive or dead or in between. I've seen that in some Pediastrum. I must admit I am jealous of the Janelia pond. My pond has a lot of Cymbella and other diatoms in it, but not so many desmids in the winter.

David

arturoag75 · Post by **arturoag75** » Mon Dec 22, 2014 3:31 am

Excellent

Jacek · Post by **Jacek** » Mon Dec 22, 2014 3:33 am

Litonotus · Post by **Litonotus** » Mon Dec 22, 2014 4:28 am

Perfect!

piermicro · Post by **piermicro** » Mon Dec 22, 2014 10:21 am

Very,very well.