Mosquito with LED light and cross polarised EPI light

[JH]

I suspect that the six radial black bars you see in each ommatidium are actually part of the lighting system of the microscope. They could be supporting structure at the back of the objective.

--Rik

Thanks Rik!

Trying to restore the eyes I kept the mosquito in a moisture environment. The alternative would have been to find a new one outside but we have had sub-zero degrees (celsius) some nights so there is not so many to be found. Luckily the eyes recovered enough for me to take another look. The radial bars are not moving when I turn the subject so they are obviously, as you suggest, part of the lighting system of the microscope.

When it comes to the green bars in the second picture above: they move with the subject when I turn it so they are not part of the lighting system. They might be (near) surface reflections of the main reflection.

In the picture below, a dark field picture taken with the BD plan 20x elwd, there is (in each lens) a circular main reflection with three radial black bars. The eight lights that surrounds each lens looks like reflections of the main reflection.

Regards Jörgen

[vasselle]

Very nice

[JH]

Thanks arturo ag75 and vasselle for looking and commenting.
This is the final (if you ever get to final) version of the mosquito head. The first one was a little shorter stack of the jpeg files that came directly out of the camera. Here I have used RAW files and adjusted the WB in LR before stacking. Curves and sharpening in PS.

Hope this can be of some use to other Adobe PS users:
I used to use Topaz Labs but I have come to use PS Smart Sharpen more and more. 100%-150% and for the pixel I use to start with a calculated value of what I think is the pixel radius of the airy disk. To get rid of some of the haze in the picture I use unsharp mask with max pixel radius (1000) and 10%. After that I reduce noise.

The second and third is a cropped version of the eyes after and before PS.


Regards Jörgen