Protos, thank you
Chris, the effect of the antiflex filter (rotatable 1/4 lamda plate placed between the objective and the subject) is just to supress flare.
The first image is taken with crossed polarizers (one at the illuminator before the beamsplitter and the analizer between the objective and the microscope head). Turning the antiflex cap you can go from typical cross pol image (almost black) to a brightfield image but with less flare. The image was taken at that "brighfiled" position. It seems that this effect is more important with lower power objectives, my antiflex is designed for a 1.25X Zeiss.
Unfortunately I didn't shot any image just with cross pol and now I've dismounted* the system and I don't plan to remount it in at least one month, but the cross pol image is pretty black because the metallic direct reflection is effectively supressed and you only see dirt, oxidation and reflections provided by scratches (maybe from some angles related with Brewster angle(?))
*I needed to dismount the stage and condenser to be able to acomodate the epi equipment, I'm designing an
open microscope with much longer clearance with parts of my macro setup and Zeiss microscopes I already have but I need to custom make a part and my machinist is always very busy.
