Desmids

Images made through a microscope. All subject types.

Moderators: rjlittlefield, ChrisR, Chris S., Pau

Olympusman
Posts: 5092
Joined: Sun Jan 15, 2012 12:31 pm

Desmids

Post by Olympusman »

I just got a new 60x dry objective and am getting the hang of it. Even with a 0.17 cover slip there is so little working distance that I have to draw excess water from under the cover slip by holding a piece of toilet paper against the edge of the slide to keep the cover slip from floating.
Here are a few desmid stack tests:

Cosmarium biretum

Image
Michael Reese Much FRMS EMS Bethlehem, Pennsylvania, USA

Olympusman
Posts: 5092
Joined: Sun Jan 15, 2012 12:31 pm

Desmids

Post by Olympusman »

Sorry -- here are the others:

Cosmarium perforatum

Image

Staurastrum armigerum

Image

Micrasterias truncata

Image
Michael Reese Much FRMS EMS Bethlehem, Pennsylvania, USA

vasselle
Posts: 1497
Joined: Sun Jan 05, 2014 2:12 am
Location: France

Post by vasselle »

Très belles séries de photo
J'aime beaucoup la sernière
Microscope Leitz Laborlux K
Boitier EOS 1200d

jswatts
Posts: 87
Joined: Mon Apr 08, 2013 11:34 pm
Location: Brisbane, Australia
Contact:

Post by jswatts »

These are great shots, Michael, especially that last Micrasterias. I've generally found when working with this sort of sample that large 22mm x 50mm coverslips are really helpful. Also, there's a bit of an art to letting just enough of the water to evaporate to suit the particular thing you're shooting: not enough and the little darlin's have too much freedom; too long and they're squished. :roll:

75RR
Posts: 766
Joined: Thu May 22, 2014 12:38 pm
Location: Estepona

Post by 75RR »

I just got a new 60x dry objective
Congratulations!
Agree with jswatts, last one very nice.
Had not foreseen the working distance limitations, good to know.

Olympusman
Posts: 5092
Joined: Sun Jan 15, 2012 12:31 pm

60x objective

Post by Olympusman »

Thanks guys.
When I bought my microtome, the retired doctor who sold it to me me threw in a couple of thousand slides and several thousand 25x40 and 25x50mm cover slips all of which are .22 mm. ( I test them with a digital caliper). This new objective requires .17 mm cover slips, but the working distance is so close that when I go from the 40x objective to the 60x, before I rotate the turret I have to drop the stage for clearance and then walk the focus into the subject. If I try to use the .22 slips, the objective strikes the slip before I can achieve focus. That said, for $60 on ebay, I can't complain and am please with the image quality.
I have an Olympus 60x that has very generous working distance but because of the lens flare, it is useless unless I stop the condenser all the way down and the image quality gets very poor -- primarily chromatic abberation.
Michael Reese Much FRMS EMS Bethlehem, Pennsylvania, USA

Post Reply Previous topicNext topic