Micrasterias mahabuleswarensis

RogelioMoreno · Post by **RogelioMoreno** » Fri Sep 12, 2014 8:00 am

I found this specimen in my sample from lake Gamboa.
I like the shape of the chloroplast.

Micrasterias mahabuleswarensis by Rogelio Moreno G., on Flickr
plan apo 20x + 1.5x, DIC, image stacking

Micrasterias mahabuleswarensis by Rogelio Moreno G., on Flickr
plan apo 20x + 1.5x, darkfield, image stacking

Micrasterias mahabuleswarensis by Rogelio Moreno G., on Flickr
plan apo 20x + 1.5x, polarized light + lambda plate, image stacking

Micrasterias mahabuleswarensis by Rogelio Moreno G., on Flickr
plan apo 20x + 1.5x, bright field, image stacking

Rogelio

vasselle · Post by **vasselle** » Fri Sep 12, 2014 9:09 am

Hello
Very nice photos
Cordialement seb..

Jacek · Post by **Jacek** » Fri Sep 12, 2014 10:07 am

fabulous

pwnell · Post by **pwnell** » Fri Sep 12, 2014 11:04 am

All of them are fabulous, especially the darkfield where you controlled the halos really well. Did you use an oil condenser? I find that I get much better results with my oil darkfield condenser and the 20x/0.75 than with the standard dry darkfield condenser.

piermicro · Post by **piermicro** » Fri Sep 12, 2014 11:05 am

Very, very nice indeed.

Ernst Hippe · Post by **Ernst Hippe** » Sat Sep 13, 2014 2:16 am

Rogelio,
this is again a marvellous set of photos!! Thank you also for including the "simple" brightfield for comparison by the poor microscopist without DIC.

Sumguy01 · Post by **Sumguy01** » Sat Sep 13, 2014 9:06 am

Great set.
Thanks for sharing.

Olympusman · Post by **Olympusman** » Sat Sep 13, 2014 10:51 am

Spectacular! I envy that scope of yours (AND your technique).

arturoag75 · Post by **arturoag75** » Sun Sep 14, 2014 1:27 am

Impressive!

Anatoly · Post by **Anatoly** » Tue Sep 16, 2014 6:55 am

Very nice!!!

jswatts · Post by **jswatts** » Tue Sep 16, 2014 8:25 pm

I really like how hard you've pushed the DIC on the first one. I don't see very many folks using optical staining, and you've used it to excellent effect. The Magenta of the background compliments the green of the chloroplasts nicely, and the contrast at the edges of the cuticle is fantastic.

Charles Krebs · Post by **Charles Krebs** » Tue Sep 16, 2014 11:13 pm

Beautiful subject and pictures.

Like them all, darkfield is my favorite.

Ecki · Post by **Ecki** » Wed Sep 17, 2014 12:19 am

Nice set.

RogelioMoreno · Post by **RogelioMoreno** » Wed Sep 17, 2014 11:58 pm

Thank you very much for your comments!

pwnell wrote: Did you use an oil condenser? I find that I get much better results with my oil darkfield condenser and the 20x/0.75 than with the standard dry darkfield condenser.

No, I used a standard dry (0.95 - 0.80) dark field condenser. I do not have the oil condenser. Lately I am doing some images without covering the specimens (I have an inverted so that is not a problem) to avoid crushing them, for example the Actinosphaerium.

Rogelio