Hydra tentacle detail

Images made through a microscope. All subject types.

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Olympusman
Posts: 5066
Joined: Sun Jan 15, 2012 12:31 pm

Hydra tentacle detail

Post by Olympusman »

A test comparing brightfield illumination and Universal Gradient Filter (UGF) illumination.
40x objective focus stacked in Zerene.

Brightfield

Image

UGF

Image
Michael Reese Much FRMS EMS Bethlehem, Pennsylvania, USA

vasselle
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Location: France

Post by vasselle »

Hello
Very nice photo
Microscope Leitz Laborlux K
Boitier EOS 1200d

Olympusman
Posts: 5066
Joined: Sun Jan 15, 2012 12:31 pm

Hydra tentacle detail

Post by Olympusman »

Vasselle,
Thanks for your kind comments for this and other posts.
Michael Reese Much FRMS EMS Bethlehem, Pennsylvania, USA

leonardturner
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Location: Atlanta, GA, USA

Post by leonardturner »

Very nice illustration of these suckers, and clearly different detail in the second example. But my ignorance is exposed; what is UGF illumination?

rjlittlefield
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Post by rjlittlefield »

leonardturner wrote:what is UGF illumination?
Universal Gradient Filter. The standard reference is http://www.photomacrography.net/forum/v ... 5832#95832 and discussion in the thread that surrounds it, "Gradient Universal Filter - 'DIC', DF, OL effects".

I don't know if Olympusman is doing exactly what's described there, or something slightly different.

--Rik

75RR
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Post by 75RR »

Thanks for the info link rjlittlefield, was working on making some darkfield/oblique stops (on acetate) over the last few days, test version Mark 2 to be printed tomorrow. Have now made up some UGF on illustrator to test as well. This makes tomorrows trip to the copy shop that much more interesting.

Olympusman
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Joined: Sun Jan 15, 2012 12:31 pm

UGF filters

Post by Olympusman »

I make my own UGF filters by shooting them with a 6X6 cm camera. I spray paint a foam core disc about 18 inches across so its half white and half black. Then I put a substage filter on the cameras ground glass to scale the disc for copy photography and shoot the disc on black and white film. When the film is processed I have a negative image which is half black and half clear. I then trim it to size for the substage filter holder on the condenser.
I have found that the results of UGF appear to be slightly crisper than straight bright field illumination, particularly with 20X and 40X objectives.
Michael Reese Much FRMS EMS Bethlehem, Pennsylvania, USA

RogelioMoreno
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Post by RogelioMoreno »

Very nice details!

Rogelio

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