Pleurotaenium sp.

RogelioMoreno · Post by **RogelioMoreno** » Mon Aug 18, 2014 7:35 am

Here a couple of Pleurotaenium sp. images.

All with Nikon TE300, plan apo 20x/0.75, Canon T3i.

Pleurotaenium sp. by Rogelio Moreno G., on Flickr
auto fluorescence (UV-2B), image stacking

Pleurotaenium sp. by Rogelio Moreno G., on Flickr
polarized light + lambda plate, image stacking

Rogelio

Jacek · Post by **Jacek** » Mon Aug 18, 2014 11:30 pm

arturoag75 · Post by **arturoag75** » Tue Aug 19, 2014 12:54 am

Beautiful

RogelioMoreno · Post by **RogelioMoreno** » Tue Aug 19, 2014 6:06 am

Thank you for your comments.

Rogelio

Ecki · Post by **Ecki** » Tue Aug 19, 2014 8:47 am

Impressive. Did you use deconvolution software?

Regards
Ecki

piermicro · Post by **piermicro** » Tue Aug 19, 2014 11:38 am

excellent as always.

RogelioMoreno · Post by **RogelioMoreno** » Tue Aug 19, 2014 12:45 pm

Thanks for your comments.

Ecki, I did not use deconvolution software, I did use ZS to stack and Detail Extractor from my Nik collection package.

Rogelio

vasselle · Post by **vasselle** » Sat Aug 23, 2014 1:49 pm

Hello
Very nice photo

carlos.uruguay · Post by **carlos.uruguay** » Sun Aug 24, 2014 8:56 am

Excellent!!

pwnell · Post by **pwnell** » Sun Aug 24, 2014 10:31 am

Really well done like always. I like how you manage to maintain a very low noise level on those fluorescent shots. I find that to not cause photobleaching from ruining the sample, I have to crank up the ISO to 800 or even 1600 at times (and use lower excitation intensity), and the PMax stacking coupled with details extractor amplifies this noise even with noise reduction.

ralfwagner · Post by **ralfwagner** » Sun Aug 24, 2014 12:58 pm

Great!

The first picture is showing 2 blue fluorescent dots at the isthmus. And, when looking closer, there is a blue fluorescence at the tips, too. Any idea what this might be?

RogelioMoreno · Post by **RogelioMoreno** » Thu Aug 28, 2014 5:48 am

Thank you for your comments.

Ralf, the cell wall emit blue light when excited with ultraviolet light.

Waldo, I used DMap on this subject.

Rogelio