Trithigmostoma steinii (ciliate)

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Protos
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Location: Lille, France

Trithigmostoma steinii (ciliate)

Post by Protos »

Found this nice Cyrtophorida ciliate in a small pond . It eats diatomae. I identified it using silver carbonate impregnation method.
Note that there is a difference in shape between live animals and stained animals. Most ublications usually favor stained animal pictures which might lead to wrong ID. This is a typical example.
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Zeiss Axiophot, transmitted and Fluorescence
BK5000, Transmitted and CP
Wild M20

Protos
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Joined: Tue Jan 07, 2014 4:01 am
Location: Lille, France

Post by Protos »

Zeiss Axiophot, transmitted and Fluorescence
BK5000, Transmitted and CP
Wild M20

piermicro
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Post by piermicro »

Nice.

Cyclops
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Post by Cyclops »

Fabulous!
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75RR
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Post by 75RR »

That is a frisky ciliate, how did you manage to take the still photographs?

Bruce Taylor
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Post by Bruce Taylor »

Very nice! The need for live and stained observations is nicely demonstrated. :) Without the fixed specimens, it might be difficult to rule out the synhymeniida.

For the stained ones, did you use the original Fernandez-Galiano protocol, or one of the updated methods? Did you have a culture to work with, or just wild specimens?
It Came from the Pond (Blog): http://www.itcamefromthepond.com/

Protos
Posts: 346
Joined: Tue Jan 07, 2014 4:01 am
Location: Lille, France

Post by Protos »

75RR wrote:That is a frisky ciliate, how did you manage to take the still photographs?
It was not that fast. I also used High speed with my Canon EOS 700D
Zeiss Axiophot, transmitted and Fluorescence
BK5000, Transmitted and CP
Wild M20

Protos
Posts: 346
Joined: Tue Jan 07, 2014 4:01 am
Location: Lille, France

Post by Protos »

Bruce Taylor wrote:Very nice! The need for live and stained observations is nicely demonstrated. :) Without the fixed specimens, it might be difficult to rule out the synhymeniida.

For the stained ones, did you use the original Fernandez-Galiano protocol, or one of the updated methods? Did you have a culture to work with, or just wild specimens?
I agree Silver staining helped a lot. I strengthens my feeling that drawing which are often based on fixed samples can be misleading.
I used Fernandez Galiano then counted the number of cilia row to differentiate from T. Cucullus
Zeiss Axiophot, transmitted and Fluorescence
BK5000, Transmitted and CP
Wild M20

Protos
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Joined: Tue Jan 07, 2014 4:01 am
Location: Lille, France

Post by Protos »

Cyclops wrote:Fabulous!
Thank you !
Zeiss Axiophot, transmitted and Fluorescence
BK5000, Transmitted and CP
Wild M20

vasselle
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Post by vasselle »

Bonjour
Superbe :smt041
Cordialement seb
Microscope Leitz Laborlux K
Boitier EOS 1200d

RogelioMoreno
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Post by RogelioMoreno »

Nice details on last one.

Rogelio

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