Thin section of an ivy sheet

[Erik]

Hello microscopists,

Today I would like to show the thin section of an ivy sheet. Despite colouring (Etzold) I show it in black-and-white, because it pleases me so better.



The photo developed with monochromatic light (551nm) and a 20x plan achromatic objective, photographs by the eyepiece (free-handle). I hope it find also with you favours.

Many greetings

Erik

http://www.mikroansichten.de

[Wim van Egmond]

Indeed much more peaceful than the normally bright colours of the coloured plant sections. With black and white it becomes a micro Blossfeldt!

Well done!

Wim

[Erik]

Hi Wim,

With a monochromatic coloring method (e.g. Heamatoxylin) show themselves details often better. In the age of Windows multicolored colouring has such as Etzold or Azan however clearly more fans.
Therefore now some more multicolored. This concerns a thin section of a bloom (Gänseblümchen,Bellis perennis), colored with Etzold:



and to compere, colored with Heamatoxylin and Eosin:



Many greetings

Erik

http://www.mikroansichten.de

[Wim van Egmond]

Nice pictures!

But I must say that I have never understood why they make stains that make plant cells look like a disco! Why pink?

I would prefer bright green finished with a touch of red!

Wim

[Erik]

Hi Wim,

"... But I must say that I have never understood why they make stains that make plant cells look like a disco! Why pink? ..."

I prefer the Eosin because it colored many components of a cell in a shiny way.

"... I would prefer bright green finished with a touch of red! ..."

You can choose the colours you prefer. I have never tried it with green, but I will do it.

Erik

[beetleman]

I like the monochromatic coloring....it gives the photo a totally different feel than normal, more 3D looking . Very sharp and lots of detail Erik.

[Wim van Egmond]

Of course a good contrast between the 2 colours is essential. But with green it looks more like the plant. I am curious which colours are possible!

best regards,

Wim

[Erik]

Sorry, but I found a mistake. The used filter in first picture was an interfencefilter with 589 nm (not 551 as described).

See the difference:

551 nm:



589 nm:



So you can see the influence of the colour of illumination.

Erik