Peacock feather (added pictures)

Images made through a microscope. All subject types.

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JH
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Peacock feather (added pictures)

Post by JH »

Peacock feather.
I wanted to try infinite lenses, realised that It was problems to use the Mitutoyo M plan apos in my Nikon Optiphot due to their length. This is a stack with the Nikon 10x TU plan Fluor (MUE12100). Canon 6D, 268 jpg:s stacked with Zerene Pmax. Curves and sharpening in Canon DPP.


Regards Jörgen

Image
Last edited by JH on Sun May 18, 2014 11:26 am, edited 1 time in total.

_Michal_
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Post by _Michal_ »

Very nice

JH
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Post by JH »

Thanks Michal

Added two pictures. The second is cropped. This time stacked tif-files, pmax, curves sharpeing and some saturation. For this type of picture I realized that it is important to turn off the Options/Preferences/Alignment/Brightness or else there is a haze all over the picture.

Regards Jörgen

Image

Image

Charles Krebs
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Post by Charles Krebs »

Nicely done. This is a great subject. Stacks pretty easily, but the lighting is very important... well done here.

JH
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Post by JH »

Thanks Charles!

This is my first try with a infinite lens. I am pleased that the Nikon 10x TU plan fluor seems to work quite well with a full frame sensor.

Regards Jörgen

carlos.uruguay
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Post by carlos.uruguay »

Very nice

rjlittlefield
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Post by rjlittlefield »

The images look very good!
JH wrote:I am pleased that the Nikon 10x TU plan fluor seems to work quite well with a full frame sensor.
This is very encouraging. I see that the MUE12100 is now offered by Edmund Optics (HERE). But its list price is showing today at $975, even more than the Mitutoyo 10X at $865. I was hoping that newer technology might bring lower prices, but I guess not yet.

How do you have your full frame camera coupled to the microscope? What is the field width at subject?

--Rik

JH
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Post by JH »

Thanks Carlos and Rik!
rjlittlefield wrote: How do you have your full frame camera coupled to the microscope?
--Rik
I use:
Dovetail: http://rafcamera.com/en/adapter-microsc ... ail-to-m42
The middle ring from M42 tube: ebay 360246527130
EOS adapter: ebay 360202866938
Almost all the extension rings from two macro extension tubes for Canon: ebay 11032124964

The tube lens is a ITL200 (ebay 111257958466) placed on a rubber o-ring inside the dovetail.

The distance between the tube lens and the microscope lens is approx. 50 mm and the distance from the tube lens to the sensor is close to 150 mm. When I tested it seemed to be more important to have the sensor / tube lens distance correct than the objective / tube lens distance.

To mount the objevctive I use a RMS to M25 thread adapter. http://rafcamera.com/en/adapters/rms-0- ... -m25-black
rjlittlefield wrote: What is the field width at subject?
--Rik
I did not measured for the first pictures -but It should have been close to 10x on sensor (I used a bellows so the distance from the tube lens to the sensor was not fixed). For the pictures below I measured the field with to be very close to 3.6 mm X 2.4 mm with the stage micrometer you tipped me about. :)

Regards Jörgen

Image
Image

rjlittlefield
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Post by rjlittlefield »

Thank you for the additional information. It sounds like optically you are using direct projection from the tube lens onto the sensor. This is an excellent configuration. I had thought that perhaps there were other relay lenses involved, so I wanted to check.

--Rik

Jacek
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Post by Jacek »

Very nice

piermicro
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Post by piermicro »

Very nice.

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