Please ID these items in this blood sample

Images made through a microscope. All subject types.

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pwnell
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Please ID these items in this blood sample

Post by pwnell »

I took this picture with a 60x objective. I am pretty sure I can ID the following correctly:

Red: White blood cells
Yellow: Red blood cells

but what are:

Green? (grey blobs)
Blue? (filamentous objects)

Image

arturoag75
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Post by arturoag75 »

Very nice...
green : white cells
ble: i suppose fibrinogen
Red: red cells with midified shape due to hosmotic process (i think)
Arturo wink:

Pau
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Post by Pau »

Waldo, another excellent quality image.

Arturo, you're 100% right (well, fibrinogen is a soluble protein, no visible microscopically, they must be fibrin, the fibrous insoluble protein filaments formed after fibrinogen modification) . There can also easily seen platelets, smaller colorless forms, in some cases associatet to fibers.
The blood is beguining to coagulate
Pau

pwnell
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Post by pwnell »

Thanks!

rjlittlefield
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Post by rjlittlefield »

Great picture, Waldo (as usual!)

For more information and a search term regarding those modified red cells, see http://en.wikipedia.org/wiki/Crenation#Red_blood_cells.

--Rik

pwnell
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Post by pwnell »

Thanks Rik, that is very informative.

vasselle
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Post by vasselle »

Excellente photo

harisA
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Post by harisA »

Great image.I have never seen a DIC blood sample image before.

Olympusman
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Blood

Post by Olympusman »

I think the reds are clusters of platelets.
Michael Reese Much FRMS EMS Bethlehem, Pennsylvania, USA

Pau
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Re: Blood

Post by Pau »

Olympusman wrote:I think the reds are clusters of platelets.
No, they are crenated erythrocytes as Arturo said. You can easily see them with your own blood: add a bit of salt concentrated dissolution or just let the slide dry a bit. I do this each year with my pupils to demonstrate osmosis. Or just google for osmosis and blood
Pau

Tom Jones
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Post by Tom Jones »

The red arrows show crenated RBC. Platelet clusters/clumps are very irregular in size and shape. In the smaller clusters, the individual platelets are almost always easily discernible.

Blue arrows show fibrin strands, and the green arrows show the WBC.

The DIC nicely shows the granulation differences between the lymphocytes (generally round and apparently agranular), and the granulocytes (neutrophils, monocytes, eosinophils and basophils). It may be an artifact, but the number of WBC appears to be abnormally high.

A very nice photo indeed.

Tom

g4lab
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Post by g4lab »

The green arrows point to white cells which come in a large variety of forms.

g4lab
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Post by g4lab »

The green arrows point to white cells which come in a large variety of forms.
aka leucocytes or leukocytes. The red cells carry oxygen and participate in clotting (along with platelets and fibrin) and the white cells go to work repairing damage, attacking invaders and taking care of certain immune matters.

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