Heliozoan

Images made through a microscope. All subject types.

Moderators: rjlittlefield, ChrisR, Chris S., Pau

Beatsy
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Heliozoan

Post by Beatsy »

From a fresh pond water sample. The main body is about 75um diameter. I think it's Raphidiophrys pallida.

Images are 20-shot focus stacks taken on a Canon 5d MkII. DIC with a 100W halogen lamp used in both. I had to delete-and-repeat a few times thanks to annoying diatoms and ciliates getting in the way or bumping the heliozoan half way through a stack set. The frustrations of shooting "live" eh :)

Plan 16x objective + 3.2x internal relay lens
Image

Neofluar 40x objective + 3.2x internal relay lens
Image

carlos.uruguay
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Post by carlos.uruguay »

Very very nice

arturoag75
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Post by arturoag75 »

Very nice!
is the 16 an achromatic objective?
arturo :wink:

Pau
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Post by Pau »

Excellent images!

I wonder if your stacks do suffer a movement artifact showing more axopodia and bubbles than the real ones as they don't stop moving while taking the frames.

Some time ago I posted a stack of a much smaller heliozoan showing this problem:
http://www.photomacrography.net/forum/v ... 235#102235
Pau

Beatsy
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Post by Beatsy »

Artruro, it's a Zeiss Plan objective. Not Apo but I think they're corrected for one more colour than a plain achromat.

Pau, I'm sure the axipodia and bubbles are all real. Surprising how many there are when not confined to a narrow focal plane. I got a couple of "broken" ones where individuals moved out of place during stacking and I spot-removed those. But the rest are in focus along their full length and only a tiny fraction is in focus in any single frame so it would be impossible to introduce a full-length "ghost" during stacking. The beast was very slowly drifting laterally, but not rolling at all (except when bumped by other critters and I restarted the stack when that happened)

Thanks all for the comments.

Olympusman
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Heliozoan

Post by Olympusman »

Nice detail on a very difficult subject to photograph. Sometimes when I encounter slow drifting or the problem with passing protists disturbing the subject, I put the camera in sequential drive mode and focus through the stack planes while holding down the remote for a rapid-fire stack.
Michael Reese Much FRMS EMS Bethlehem, Pennsylvania, USA

piermicro
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Post by piermicro »

very nice

arturoag75
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Post by arturoag75 »

Beatsy wrote:Artruro, it's a Zeiss Plan objective. Not Apo but I think they're corrected for one more colour than a plain achromat.
.
thanks...simple and very useful lens :wink:

Jacek
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Post by Jacek »

Very nice

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