Pennate diatom, 60X DIC, 100X darkfield

Images made through a microscope. All subject types.

Moderators: rjlittlefield, ChrisR, Chris S., Pau

Charles Krebs
Posts: 5865
Joined: Tue Aug 01, 2006 8:02 pm
Location: Issaquah, WA USA
Contact:

Pennate diatom, 60X DIC, 100X darkfield

Post by Charles Krebs »

After taking a few DIC shots of these diatoms with the 60X, I thought I would try some high magnification darkfield with the 100X. Usually a pain and messy with the oil. And frustrating with live diatoms because at these magnifications they seem to move realatively quickly. But I think it gave a much nicer rendition and "feel" for the the glass-like clear silica frustule.


Image

Image

Image

Beatsy
Posts: 1840
Joined: Fri Jul 05, 2013 3:10 am
Location: Malvern, UK

Post by Beatsy »

Beautiful at 100x. How deep did you stack these? I've never bothered to try such high mag imaging on these because they positively zoom along as you say, and I don't have flash set up yet. But you've inspired me to get a move on and do so :)

abpho
Posts: 1508
Joined: Wed Aug 17, 2011 7:11 pm
Location: Earth

Post by abpho »

Wow Charles. These are stunning.
I'm in Canada! Isn't that weird?

Charles Krebs
Posts: 5865
Joined: Tue Aug 01, 2006 8:02 pm
Location: Issaquah, WA USA
Contact:

Post by Charles Krebs »

Beatsy,
How deep did you stack these?
Only 6 images in each of the 100X stacks. These were done without flash... using the "stock" 100W halogen lamphouse. Typically I keep an eye on a few really nice looking diatoms as the water evaporates from under the cover-slip. Then just as soon as the slip drops down enough that they get "stuck", I shoot the frames I need as quickly as possible before they frustule cracks from cover-glass pressure.

arturoag75
Posts: 1600
Joined: Fri Feb 05, 2010 1:05 am
Location: italy
Contact:

Post by arturoag75 »

Amazing job as usual :shock: :wink:

Jacek
Posts: 5354
Joined: Sun Oct 02, 2011 7:00 am
Location: Poland

Post by Jacek »

excellent photos :shock:

Beatsy
Posts: 1840
Joined: Fri Jul 05, 2013 3:10 am
Location: Malvern, UK

Post by Beatsy »

Then just as soon as the slip drops down enough that they get "stuck", I shoot the frames...
Doh! I never thought of that. I usually run a thin bead of vaseline around my cover slips to prevent evaporation. Surprising how long things keep going too. Thanks for the tip.[/quote]

sebba28
Posts: 298
Joined: Thu Dec 29, 2011 11:13 pm
Location: Poland,Ireland

Post by sebba28 »

Excellent shots. =D>

Litonotus
Posts: 1394
Joined: Mon Jun 20, 2011 1:48 am
Location: Poland
Contact:

Post by Litonotus »

great to see you posting again (:
my FB page

I'm looking for the the extemely rare V-IM magnification changer for the E800 scope. If you have seen a listing or have one for sale please let me know.

piermicro
Posts: 861
Joined: Mon Mar 10, 2014 7:52 am
Location: ITALY
Contact:

Post by piermicro »

These are stunning.

carlos.uruguay
Posts: 5356
Joined: Thu Feb 23, 2012 8:05 pm
Location: Uruguay - Montevideo - America del Sur
Contact:

Post by carlos.uruguay »

Wonderful photos!!!!

Olympusman
Posts: 4878
Joined: Sun Jan 15, 2012 12:31 pm

Diatoms

Post by Olympusman »

Absolutley beautiful.
Michael Reese Much FRMS EMS Bethlehem, Pennsylvania, USA

vasselle
Posts: 1497
Joined: Sun Jan 05, 2014 2:12 am
Location: France

Post by vasselle »

Magnifique photos

Sumguy01
Posts: 1687
Joined: Mon Jan 28, 2013 11:05 pm
Location: Ketchikan Alaska USA

Post by Sumguy01 »

:D :smt038 :smt041 Hi Charles.
So glad you are back at it.
Great pics.
Thanks for sharing

Tom Jones
Posts: 313
Joined: Sat Jan 31, 2009 2:05 am
Location: Crestline, CA

Post by Tom Jones »

Insufficient superlatives...

Tom

Post Reply Previous topicNext topic